IL12B ELISA Kit

Catalog No. ABIN5026926

Product Details IL12B ELISA Kit

Target: IL12B (Interleukin 12 beta (IL12B))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 31.2-2000 pg/mL

Minimum Detection Limit: 31.2 pg/mL

Application: ELISA

Purpose: This immunoassay kit allows for the in vitro quantitative determination of Human IL-12 concentrations in serum, plasma and other biological fluids.

Sample Type: Plasma, Serum

Analytical Method: Quantitative

Sensitivity: < 18.7 pg/mL

Components: plate, standard, diluent, antibodies

Material not included: pipettes, tubes, reader, incubator

Application Details

Sample Volume: 100 μL

Assay Time: 3 - 4 h

Plate: Pre-coated

Protocol: This assay employs an antibody specific for Anti-human IL-12 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-12 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-humanIL-12 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-12 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Restrictions: For Research Use only

Handling

Precaution of Use: protect your eyes

Storage: 4 °C

Target Details for IL12B

Target: IL12B (Interleukin 12 beta (IL12B))

Alternative Name: IL-12p40 (Interleukin 12p40) (IL12B Products)

Synonyms: CLMF ELISA Kit, CLMF2 ELISA Kit, IL-12B ELISA Kit, NKSF ELISA Kit, NKSF2 ELISA Kit, p40 ELISA Kit, Il-12b ELISA Kit, Il12p40 ELISA Kit, Il-12p40 ELISA Kit, Il12 ELISA Kit, IL-12p40 ELISA Kit, IL-12 ELISA Kit, IL-12 p40 ELISA Kit, il12b ELISA Kit, il12p40.b ELISA Kit, zgc:152789 ELISA Kit, IL-12.p40 ELISA Kit, IL12p40 ELISA Kit, IL12B ELISA Kit, CLMF p40 ELISA Kit, interleukin 12B ELISA Kit, interleukin 12b ELISA Kit, interleukin 12Ba ELISA Kit, IL12B ELISA Kit, Il12b ELISA Kit, il12ba ELISA Kit

Pathways: JAK-STAT Signaling, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation