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Transferrin Receptor ELISA Kit

TFRC Reactivity: Human Colorimetric Sandwich ELISA Cell Culture Lysate, Plasma, Serum
Catalog No. ABIN5067899
  • Target See all Transferrin Receptor (TFRC) ELISA Kits
    Transferrin Receptor (TFRC) (Transferrin Receptor (p90, CD71) (TFRC))
    Reactivity
    • 5
    • 5
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Application
    ELISA
    Purpose
    Human sTfR ELISA is a sandwich enzyme immunoassay for the quantitative measurement of soluble human transferrin receptor in serum, plasma and tissue culture medium.
    Sample Type
    Cell Culture Lysate, Plasma, Serum
    Analytical Method
    Quantitative
    Sensitivity
    2 ng/mL
    Characteristics
    Transferrin Receptor, soluble, Human, ELISA Kit (TfR1, TFRC, CD71, TR, Tfr, TfnrR, sTfR)
    Components
    • Microtiter Strips, 1x 96 wells, coated with anti-Hu sTfR Mab
    • Mab (HRP), 1x13ml
    • Standard, 2ug/ml, 1x100ul
    • Standard, 1ug/ml, 1x100ul
    • Standard, 0.5ug/ml, 1x.100ul
    • Standard, 0.2ug/ml, 1x100ul
    • Standard, 0.1ug/ml, 1x100ul
    • Standard, 0.05ug/ml, 1x100ul
    • Quality Control, High, 50X, (5..3ug /ml), 1x50ul
    • Quality Control, Low, 50X, (1..0ug/ml), 1x50ul
    • Dilution Buffer, 2x13ml
    • Wash Solution, 10X, 1x100ml
    • Substrate Solution (TMB), 1x13ml
    • Stop Solution (0.2M O4), 1x13ml
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  • Plate
    Pre-coated
    Protocol
    Principle:
    • Standards, quality controls and samples of sera are incubated in microtitration wells coated with monoclonal anti-human sTfR antibody. After a thorough wash, monoclonal anti-human sTfR antibody labeled with horseradish peroxidase (HRP) is added to the wells and incubated with the immobilized antibody'sTfR complex. Following another washing step, the remaining HRP-conjugated antibody is allowed to react with the substrate H2O2 and tetramethylbenzidine. The reaction is stopped by addition of acidic solution, and absorbance of the resulting yellow color product is measured spectrophotometrically at 450nm. The absorbance is proportional to the concentration of sTfR. A standard curve is constructed by plotting absorbance values versus recombinant sTfR concentrations of standards, and concentrations of unknown samples are determined using this standard curve.
    Restrictions
    For Research Use only
  • Storage
    4 °C
    Storage Comment
    4°C
  • Target See all Transferrin Receptor (TFRC) ELISA Kits
    Transferrin Receptor (TFRC) (Transferrin Receptor (p90, CD71) (TFRC))
    Alternative Name
    Transferrin Receptor (TFRC Products)
    Synonyms
    Trfr ELISA Kit, LOC100219280 ELISA Kit, tfr ELISA Kit, cd71 ELISA Kit, tfr1 ELISA Kit, trfr ELISA Kit, CD71 ELISA Kit, T9 ELISA Kit, TFR ELISA Kit, TFR1 ELISA Kit, TR ELISA Kit, TRFR ELISA Kit, p90 ELISA Kit, 2610028K12Rik ELISA Kit, AI195355 ELISA Kit, AI426448 ELISA Kit, AU015758 ELISA Kit, E430033M20Rik ELISA Kit, Mtvr-1 ELISA Kit, Mtvr1 ELISA Kit, transferrin receptor ELISA Kit, TFRC ELISA Kit, Tfrc ELISA Kit, tfrc ELISA Kit
    Pathways
    Transition Metal Ion Homeostasis
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