C4A ELISA Kit

Catalog No. ABIN5067922

Product Details C4A ELISA Kit

Target: C4A (C4a) (Complement Fragment C4a (C4a))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Purpose: The Complement C4-A (C4A) ELISA Kit (Human) is a quantitative sandwich assay for the detection of C4A in human serum, plasma, tissue homogenates and other biological fluids.

Sample Type: Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Sensitivity: > 0.938 ng/mL

Characteristics: Complement C4-A (C4A) ELISA Kit (Human)

Components:

• *354394A: Microtiter Strips, 8x12 wells.
• *354394B: Standard, 2 vials
• 354394C: Sample/Standard Dilution Buffer, 1x20ml
• 354394D: Antibody-Biotin (Concentrated), 1x120ul
• 354394E: Antibody Dilution Buffer, 1x10ml
• 354394F: Streptavidin-HRP Conjugate (SABC), 1x120ul
• 354394G: SABC Dilution Buffer, 1x10ml
• 354394H: TMB Substrate, 1x10ml
• 354394J: Stop Solution, 1x10ml
• 354394K: Wash Buffer (25X), 1x30ml

Application Details

Plate: Pre-coated

Protocol: Principle:

• The microtiter plate provided in this kit has been pre-coated with an antibody specific to C4A. Standards and samples are added to the appropriate microtiter plate wells followed by a biotin-conjugated antibody specific to C4A. Streptavidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain C4A, biotin-conjugated antibody and enzyme-conjugated Streptavidin complex will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulfuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of C4A in the sample is then determined by comparing the O.D. of the sample to the standard curve.

Assay Precision: Precision:

• Intra-Assay CV: <8%
• Inter-Assay CV: <10%

Restrictions: For Research Use only

Handling

Storage: 4 °C,-20 °C

Storage Comment: 4°C/-20°C

Target Details for C4A

Target: C4A (C4a) (Complement Fragment C4a (C4a))

Alternative Name: Complement C4-A (C4A) (C4a Products)

Synonyms: C4 ELISA Kit, C4A2 ELISA Kit, C4A3 ELISA Kit, C4A4 ELISA Kit, C4A6 ELISA Kit, C4AD ELISA Kit, C4S ELISA Kit, CO4 ELISA Kit, CPAMD2 ELISA Kit, RG ELISA Kit, Slp ELISA Kit, C4-1 ELISA Kit, C4b ELISA Kit, complement C4A (Rodgers blood group) ELISA Kit, complement component 4A (Rodgers blood group) ELISA Kit, C4A ELISA Kit, C4a ELISA Kit

Pathways: Complement System