CYP3A4 ELISA Kit

Catalog No. ABIN5067926

Product Details CYP3A4 ELISA Kit

Target: CYP3A4 (Cytochrome P450, Family 3, Subfamily A, Polypeptide 4 (CYP3A4))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Purpose: The CYP3A4 (Cytochrome P450 3A4) ELISA Kit (Human) is a quantitative sandwich assay for the detection of CYP3A4 in human serum, plasma, tissue homogenates and other biological fluids.

Sample Type: Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Sensitivity: > 46.9 pg/mL

Characteristics: CYP3A4 (Cytochrome P450 3A4) ELISA Kit (Human)

Components:

• *355152A: Microtiter Strips, 8x12 wells.
• *355152B: Standard, 2 vials
• 355152C: Sample/Standard Dilution Buffer, 1x20ml
• 355152D: Antibody-Biotin (Concentrated), 1x120ul
• 355152E: Antibody Dilution Buffer, 1x10ml
• 355152F: Streptavidin-HRP Conjugate (SABC), 1x120ul
• 355152G: SABC Dilution Buffer, 1x10ml
• 355152H: TMB Substrate, 1x10ml
• 355152J: Stop Solution, 1x10ml
• 355152K: Wash Buffer (25X), 1x30ml

Application Details

Plate: Pre-coated

Protocol: Principle:

• The microtiter plate provided in this kit has been pre-coated with an antibody specific to CYP3A4. Standards and samples are added to the appropriate microtiter plate wells followed by a biotin-conjugated antibody specific to CYP3A4. Streptavidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain CYP3A4, Biotin-conjugated antibody and enzyme-conjugated Streptavidin complex will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulfuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of CYP3A4 in the sample is then determined by comparing the O.D. of the sample to the standard curve.

Assay Precision: Precision:

• Intra-Assay CV: <8%
• Inter-Assay CV: <10%

Restrictions: For Research Use only

Handling

Storage: 4 °C,-20 °C

Storage Comment: 4°C/-20°C

Target Details for CYP3A4

Target: CYP3A4 (Cytochrome P450, Family 3, Subfamily A, Polypeptide 4 (CYP3A4))

Alternative Name: CYP3A4 (Cytochrome P450 3A4) (CYP3A4 Products)

Synonyms: CP33 ELISA Kit, CP34 ELISA Kit, CYP3A ELISA Kit, CYP3A3 ELISA Kit, CYPIIIA3 ELISA Kit, CYPIIIA4 ELISA Kit, HLP ELISA Kit, NF-25 ELISA Kit, P450C3 ELISA Kit, P450PCN1 ELISA Kit, MGC108372 ELISA Kit, CYP3A80 ELISA Kit, CYP3A4 ELISA Kit, CYP3A21 ELISA Kit, CYPIIIA21 ELISA Kit, CYP3A12 ELISA Kit, cytochrome P450 family 3 subfamily A member 4 ELISA Kit, cytochrome P450 family 3 subfamily A member 43 ELISA Kit, cytochrome P450 3A4 ELISA Kit, cytochrome P450, subfamily IIIA, polypeptide 4 ELISA Kit, cytochrome P450, subfamily IIIA (niphedipine oxidase), polypeptide 4 ELISA Kit, cytochrome P450, family 3, subfamily A, polypeptide 4 ELISA Kit, CYP3A4 ELISA Kit, CYP3A43 ELISA Kit, PTRG_01782 ELISA Kit, PTRG_06060 ELISA Kit, cyp3a4 ELISA Kit

Pathways: Steroid Hormone Biosynthesis