HMGB1 ELISA Kit

Catalog No. ABIN5067936

Product Details HMGB1 ELISA Kit

Target: HMGB1 (High Mobility Group Box 1 (HMGB1))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Purpose: The Hmgb1 (High mobility group protein B1) ELISA Kit is a quantitative sandwich assay for the detection of Hmgb1 in mouse serum, plasma, tissue homogenates and other biological fluids.

Sample Type: Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Sensitivity: 9.375 pg/mL

Characteristics: Hmgb1 (High mobility group protein B1) ELISA Kit (Mouse)

Components:

• *356299A: Microtiter Strips, 1x96 wells (8x12 wells).
• *356299B: Standard, 2x1 vial
• 356299C: Sample/Standard Dilution Buffer, 1x20ml
• 356299D: Antibody (Biotin) (Concentrated), 1x120ul
• 356299E: Antibody Dilution Buffer, 1x10ml
• 356299F: Streptavidin (HRP) (SABC), 1x120ul
• 356299G: SABC Dilution Buffer, 1x10ml
• 356299H: TMB Substrate, 1x10ml
• 356299J: Stop Solution, 1x10ml
• 356299K: Wash Buffer, 25X, 1x30ml

Application Details

Plate: Pre-coated

Protocol: Principle:

• This ELISA kit employs the sandwich enzyme-linked immunoassay technique, utilizing a microtiter plate provided in this kit has been pre-coated with an antibody specific to Hmgb1. Streptavidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Hmgb1, Biotin-conjugated antibody and enzyme-conjugated Streptavidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulfuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Hmgb1 in the sample is then determined by comparing the O.D. of the sample to the standard curve.

Assay Precision: Precision:

• Intra-assay CV: <8%
• Inter-assay CV: <10%

Restrictions: For Research Use only

Handling

Storage: 4 °C,-20 °C

Storage Comment: 4°C/-20°C

Target Details for HMGB1

Target: HMGB1 (High Mobility Group Box 1 (HMGB1))

Alternative Name: Hmgb1 (High mobility group protein B1) (HMGB1 Products)

Synonyms: HMG1 ELISA Kit, HMG3 ELISA Kit, SBP-1 ELISA Kit, DEF ELISA Kit, HMG-1 ELISA Kit, Hmg1 ELISA Kit, amphoterin ELISA Kit, p30 ELISA Kit, hmgb1 ELISA Kit, ik:tdsubc_1a5 ELISA Kit, wu:fb23c02 ELISA Kit, xx:tdsubc_1a5 ELISA Kit, zgc:56110 ELISA Kit, zgc:77104 ELISA Kit, hmg-1 ELISA Kit, hmg3 ELISA Kit, sbp-1 ELISA Kit, hmg1 ELISA Kit, HMGB1 ELISA Kit, Ac2-008 ELISA Kit, high mobility group box 1 ELISA Kit, high-mobility group box 1 ELISA Kit, high mobility group box 1a ELISA Kit, high mobility group box 1 L homeolog ELISA Kit, high mobility group protein B1 ELISA Kit, HMGB1 ELISA Kit, Hmgb1 ELISA Kit, hmgb1 ELISA Kit, hmgb1a ELISA Kit, hmgb1.L ELISA Kit, LOC100359149 ELISA Kit

Pathways: p53 Signaling, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Toll-Like Receptors Cascades, Smooth Muscle Cell Migration, Inflammasome