Calreticulin ELISA Kit

Catalog No. ABIN5067947

Product Details Calreticulin ELISA Kit

Target: Calreticulin (CALR)

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Purpose: The Human Calreticulin (CALR) ELISA Kit is a quantitative sandwich assay for the detection of CALR in human serum, plasma, tissue homogenates and other biological fluids.

Sample Type: Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Sensitivity: 0.094 ng/mL

Characteristics: Calreticulin (CALR) ELISA Kit (Human)

Components:

• *354460A: Microtiter Strips, 8x12 wells.
• *354460B: Standard, 2 vials
• 354460C: Sample/Standard Dilution Buffer, 1x20ml
• 354460D: Antibody-Biotin (Concentrated), 1x120ul
• 354460E: Antibody Dilution Buffer, 1x10ml
• 354460F: Streptavidin-HRP Conjugate (SABC), 1x120ul
• 354460G: SABC Dilution Buffer, 1x10ml
• 354460H: TMB Substrate, 1x10ml
• 354460J: Stop Solution, 1x10ml
• 354460K: Wash Buffer (25X), 1x30ml

Application Details

Plate: Pre-coated

Protocol: Principle:

• The microtiter plate provided in this kit has been pre-coated with an antibody specific to CALR. Standards and samples are added to the appropriate microtiter plate wells followed by a biotin-conjugated antibody specific to CALR. Streptavidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain CALR, Biotin-conjugated antibody and enzyme-conjugated Streptavidin complex will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulfuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of CALR in the sample is then determined by comparing the O.D. of the sample to the standard curve.

Assay Precision: Precision:

• Intra-Assay CV: <8%
• Inter-Assay CV: <10%

Restrictions: For Research Use only

Handling

Storage: 4 °C,-20 °C

Storage Comment: 4°C/-20°C

Target Details for Calreticulin

Target: Calreticulin (CALR)

Abstract: CALR Products

Synonyms: CRT ELISA Kit, RO ELISA Kit, SSA ELISA Kit, cC1qR ELISA Kit, crc ELISA Kit, crt ELISA Kit, Calregulin ELISA Kit, CG9429 ELISA Kit, Clr ELISA Kit, Dmel\\CG9429 ELISA Kit, anon-EST:Liang-2.11 ELISA Kit, anon-EST:Liang-2.17 ELISA Kit, anon-EST:fe3D7 ELISA Kit, clone 2.11 ELISA Kit, clone 2.17 ELISA Kit, eth ELISA Kit, potp ELISA Kit, cc1qr ELISA Kit, ssa ELISA Kit, NV19007 ELISA Kit, CRP55 ELISA Kit, ERp60 ELISA Kit, HACBP ELISA Kit, CHOT1 ELISA Kit, CHT1 ELISA Kit, CT1 ELISA Kit, AA589632 ELISA Kit, CRTR ELISA Kit, Creat ELISA Kit, CCDS1 ELISA Kit, calrl2 ELISA Kit, fc66d10 ELISA Kit, wu:fc66d10 ELISA Kit, wu:fj50c03 ELISA Kit, zgc:66153 ELISA Kit, calreticulin ELISA Kit, calreticulin L homeolog ELISA Kit, Calreticulin ELISA Kit, putative calreticulin ELISA Kit, solute carrier family 6 member 8 ELISA Kit, solute carrier family 6 (neurotransmitter transporter, creatine), member 8 ELISA Kit, CALR ELISA Kit, calr.L ELISA Kit, Crt ELISA Kit, crt ELISA Kit, CRT ELISA Kit, Calr ELISA Kit, calr ELISA Kit, LMJF_31_2600 ELISA Kit, Crc ELISA Kit, Slc6a8 ELISA Kit, SLC6A8 ELISA Kit, LOC8269812 ELISA Kit

UniProt: P27797

Pathways: Retinoic Acid Receptor Signaling Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Nuclear Hormone Receptor Binding, ER-Nucleus Signaling, Unfolded Protein Response