IL12 ELISA Kit

Catalog No. ABIN5068331

Product Details IL12 ELISA Kit

Target: IL12 (Interleukin 12 (IL12))

Reactivity: Rat

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Sample Type: Plasma, Cell Culture Supernatant, Serum, Tissue Samples

Sensitivity: 7 pg/mL

Characteristics: IL-12 p70 ELISA Kit (Rat) (Interleukin-12 subunit alpha, IL-12A, Cytotoxic lymphocyte maturation factor 35kD subunit, CLMF p35, IL-12 subunit p35, NK cell stimulatory factor chain 1, IL12A, NKSF1, Interleukin-12 subunit beta, IL-12B, Cytotoxic lymphocyte maturation factor 40kD)

Components:

• Aluminium pouches with a Microwell Plate: 8x12
• Standard: 2xVial
• Biotinylated antibody: 2xVial
• Streptavidin-HRP: 2xVial
• Assay Buffer (25ml/Vial): 2xVial
• Wash Buffer Concentrate 20x (30ml/Vial): 1xVial
• TMB Substrate Solution: 1x12ml
• Stop Solution (12ml/Vial): 1xVial
• Plate Covers- Adhesive strips: 3xstrips
• Instruction: 1x

Application Details

Plate: Pre-coated

Protocol: Principle:

• This assay employs the Sandwich immunoassay technique. An anti-r IL-12 p70 monoclonal coating antibody is adsorbed onto microwells. IL-12 p70 present in the sample or standard binds to antibodies adsorbed to the microwells. Following incubation unbound sample or standard are removed during a wash step. a Biotinylated anti-r IL-12 p70 antibody is added and binds to IL-12 p70 captured by the first antibody. Following incubation unbound Biotinylated anti-r IL-12 p70 antibody is removed during a wash step. A Streptavidin-HRP is added and binds to Biotinylated anti-r IL-12 p70 antibody. Following incubation unbound Streptavidin-HRP is removed during a wash step. A colored product is formed in proportion to the amount of IL-12 p70 present in the sample. The reaction is terminated by addition of acid and absorbance is measured at 450nm. A standard curve is prepared from seven IL-12 p70 standard dilutions and IL-12 p70 sample concentration determined.

Restrictions: For Research Use only

Handling

Storage: 4 °C,-20 °C

Storage Comment: 4°C/-20°C

Target Details for IL12

Target: IL12 (Interleukin 12 (IL12))

Alternative Name: IL-12 p70 (IL12 Products)

Synonyms: CLMF ELISA Kit, CLMF2 ELISA Kit, IL-12B ELISA Kit, NKSF ELISA Kit, NKSF2 ELISA Kit, p40 ELISA Kit, Il-12b ELISA Kit, Il12p40 ELISA Kit, Il-12p40 ELISA Kit, Il12 ELISA Kit, IL-12p40 ELISA Kit, IL-12 ELISA Kit, LOC100217488 ELISA Kit, IL-12p35 ELISA Kit, Il-12a ELISA Kit, Ll12a ELISA Kit, p35 ELISA Kit, IL12p40 ELISA Kit, IL12B ELISA Kit, IL-12 p35 ELISA Kit, interleukin 12B ELISA Kit, interleukin 12A ELISA Kit, interleukin 12b ELISA Kit, interleukin 12a ELISA Kit, Interleukin 12 level ELISA Kit, IL12B ELISA Kit, IL12A ELISA Kit, Il12b ELISA Kit, Il12a ELISA Kit, IL12 ELISA Kit, il12a ELISA Kit

Background: Interleukin 12 (IL-12), also known as natural killer cell stimulatory factor (NKSF) or cytotoxic lymphocyte maturation factor (CLMF), is a heterodimeric pleiotropic cytokine made up of a 40kD (p40) subunit and a 35kD (p35) subunit. The IL-12 p40 subunit is shared by IL-23, another heterodimeric cytokine that has biological activities similar to, as well as distinct from, IL-12. IL-12 is produced by macrophages and B cells and has been shown to have multiple effects on T cells and natural killer (NK) cells. While mouse IL-12 is active on both human and mouse cells, human IL-12 is not active on mouse cells.

Pathways: JAK-STAT Signaling, TLR Signaling, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation, Cancer Immune Checkpoints, Inflammasome