GFP VHH, recombinant binding protein (GFP-Trap) Kit
- Antibody Type
- Recombinant Antibody
- single-domain Antibody (sdAb)
- Aequorea victoria
- Camelid (Camelidae)
- Affinity Measurement (AM), Chromatin Immunoprecipitation (ChIP), Enzyme Activity Assay (EAA), Immunoprecipitation (IP), Mass Spectrometry (MS), Protein Complex Immunoprecipitation (Co-IP), Pull-Down Assay (Pull-Down), Purification (Purif)
- For biochemical analyses of GFP fusion proteins.
- Sample Type
- Cell Extracts
- Cross-Reactivity (Details)
- GFP-Trap® specifically binds to eGFP, wtGFP, GFP S65T, TagGFP, eYFP, YFP, Venus, Citrin, CFP. No binding to proteins derived from DsRed, all RFPs and TurboGFP can be detected.
Antibodies - extremely powerful tools in biomedical research - are large complex molecules (~ 150 kDa) consisting of two heavy and two light chains. Due to their complex structure, the use of antibodies is often limited and hindered by batch-to-batch variations.
Camelidae (camels, dromedaries, llamas and alpacas) possess functional antibodies devoid of light chains, so-called heavy chain antibodies (hcAbs). hcAbs recognize and bind their antigens via a single variable domain (VHH). These VHH domains are the smallest intact antigen binding fragments (~ 13 kDa).
Nano-Traps are based on single domain antibody fragments (VHHs) derived from alpaca.
- Purified protein
- Alpaca anti-GFP VHH, purified unconjugated protein
- Material not included
- Lysis buffer (CoIP), 10x RIPA buffer, Dilution buffer, Wash buffer, Elution buffer
- Application Notes
- Green fluorescent proteins (GFP) and variants thereof are widely used to study protein localization and dynamics. For biochemical analyses including mass spectroscopy and enzyme activity measurements these GFP-fusion proteins and their interacting factors can be isolated fast and efficiently (one step) via Immunoprecipitation using the GFP-Trap®. The GFP-Trap®_A enables purification of any protein of interest fused to GFP.
- Assay Time
- 1.5 h
- Robust and versatile tool for biochemical analyses of GFP-fusion proteins
- Short incubation times (5 - 30 min)
- Quantitative isolation of fusion proteins and transiently bound factors from cell extracts or organelles
- Low unspecific binding
- No contaminating heavy and light chains of conventional antibodies
- Applicable in Chromatin Immunoprecipitation (ChIP)
- For Research Use only
- 250 µL resin (1mg/mL)
- 1 x PBS,0.01% Sodium azide
- Sodium azide
- Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Do not freeze.
- 4 °C
- Expiry Date
- 12 months
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- Morra, Del Carratore, Muhamadali, Horga, Halliwell, Goodacre, Breitling, Dixon: "Translation Stress Positively Regulates MscL-Dependent Excretion of Cytoplasmic Proteins." in: mBio, Vol. 9, Issue 1, 2019 (PubMed).
- Alternative Name
- green fluorescent protein, gfp
- The green fluorescent protein (GFP) and variants thereof are widely used to study the subcellular localization and dynamics of proteins. GFP fusion proteins can be expressed in different cell types at different expression levels by transient or stable transfection. Transient expression may provide quick informative results, however, in many cases it is necessary to generate stable cell lines that express the GFP fusion protein of interest at a level similar to the one of the endogenous protein. Quantification of GFP fusion proteins in cells can be tricky since existing methods, like fluorescence microscopy or Western Blotting, are often shows insufficient signal to noise ratios or high signal variabilities .