Glutathione Peroxidase 1 ELISA Kit

Catalog No. ABIN5519268

Product Details Glutathione Peroxidase 1 ELISA Kit

Target: Glutathione Peroxidase 1 (GPX1)

Reactivity: Rat

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 31.25 pg/mL - 2000 pg/mL

Minimum Detection Limit: 31.25 pg/mL

Application: ELISA

Purpose: The kit is a sandwich enzyme immunoassay technique for the in vitro quantitative measurement in various sample types.

Sample Type: Plasma, Serum

Analytical Method: Quantitative

Sensitivity: 18.75 pg/mL

Components:

• Pre-coated, ready to use 96-well strip plate, flat buttom
• Plate sealer for 96 wells
• Reference Standard
• Reference Standard & Sample Diluent
• Biotinylated Detection Antibody (100 x concentrate)
• HRP Conjugate (100 x concentrate)
• Biotinylated Detection Antibody Diluent
• HRP Conjugate Diluent
• Substrate Reagent
• Stop Solution
• Wash Buffer (25 x concentrate)
• Instruction manual

Application Details

Sample Volume: 100 µL

Assay Time: 1 - 4.5 h

Plate: Pre-coated

Protocol:

1. Add 100 µL standard or sample to each well. Incubate for 90 min at 37 °C.
2. Remove the liquid. Add 100 µL Biotinylated Detection Antibody. Incubate for 1 hour at 37 °C.
3. Aspirate and wash 3 times.
4. Add 100 µL HRP Conjugate. Incubate for 30 min at 37 °C.
5. Aspirate and wash 5 times.
6. Add 90 µL Substrate Reagent. Incubate for 15 min at 37 °C.
7. Add 50 µL Stop Solution. Read at 450 nm immediately.
8. Calculation of results.

Reagent Preparation:

1. Bring all reagents to room temperature (18~25 °C) before use. Follow the Microplate reader manual for set-up and preheat it for 15 min before OD measurement.
2. Wash Buffer: Dilute 30 mL of Concentrated Wash Buffer with 720 mL of deionized or distilled water to prepare 750 mL of Wash Buffer.Note: if crystals have formed in the concentrate, warm it in a 40 °C water bath and mix it gently until the crystals have completely dissolved
3. Standard working solution: Centrifuge the standard at 10,000xg for 1 min. Add 1.0 mL of Reference Standard &Sample Diluent, let it stand for 10 min and invert it gently several times. After it dissolves fully, mix it thoroughly with a pipette. This reconstitution produces a working solution of 2000 pg/mL. Then make serial dilutions as needed. The recommended dilution gradient is as follows: 2000, 1000, 500, 250, 125, 62.5, 31.25, 0 pg/mL. Dilution method: Take 7 EP tubes, add 500 μLof Reference Standard & Sample Diluent to each tube. Pipette 500 μLof the 2000 pg/mL working solution to the first tube and mix up to produce a 1000 pg/mL working solution. Pipette 500 μLof the solution from the former tube into the latter one according to these steps. The illustration below is for reference. Note: the last tube is regarded as a blank. Don't pipette solution into it from the former tube.
4. Biotinylated Detection Antibody working solution: Calculate the required amount before the experiment (100 μL/well). In preparation, slightly more than calculated should be prepared. Centrifuge the stock tube before use, dilute the 100x Concentrated Biotinylated Detection Antibody to 1xworking solution with Biotinylated Detection Antibody Diluent.
5. Concentrated HRP Conjugate working solution: Calculate the required amount before the experiment (100 μL/well). In preparation, slightly more than calculated should be prepared. Dilute the 100x Concentrated HRP Conjugate to 1x working solution with Concentrated HRP Conjugate Diluent.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Storage Comment: The unopened kit can be stored at 4℃ for 1 month. If the kit is not used within 1 month, store the items separately according to the conditions since the kit is received.

Target Details for Glutathione Peroxidase 1

Target: Glutathione Peroxidase 1 (GPX1)

Alternative Name: GPX1(Glutathione Peroxidase 1) (GPX1 Products)

Synonyms: Gpx ELISA Kit, ATGPX1 ELISA Kit, F13D4.40 ELISA Kit, F13D4_40 ELISA Kit, GLUTATHIONE PEROXIDASE 1 ELISA Kit, glutathione peroxidase 1 ELISA Kit, gshpx1 ELISA Kit, AI195024 ELISA Kit, AL033363 ELISA Kit, CGPx ELISA Kit, GPx-1 ELISA Kit, GSHPx-1 ELISA Kit, GPX1 ELISA Kit, gpx1 ELISA Kit, cb690 ELISA Kit, zgc:92737 ELISA Kit, zgc:103683 ELISA Kit, GPXD ELISA Kit, GSHPX1 ELISA Kit, GSHPx ELISA Kit, wu:fd42f12 ELISA Kit, wu:fj22d06 ELISA Kit, zgc:101700 ELISA Kit, glutathione peroxidase Gpx1 ELISA Kit, glutathione peroxidase ELISA Kit, glutathione peroxidase 1 ELISA Kit, glutathione peroxidase 1a ELISA Kit, glutathione peroxidase family protein ELISA Kit, proliferating cell nuclear antigen ELISA Kit, glutathione peroxidase 1b ELISA Kit, glutathione peroxidase 1 S homeolog ELISA Kit, gpx1 ELISA Kit, GPX1 ELISA Kit, gpx ELISA Kit, Gpx ELISA Kit, Gpx1 ELISA Kit, gpx1a ELISA Kit, POPTR_0003s12620g ELISA Kit, Pcna ELISA Kit, LBL_RS00590 ELISA Kit, btuE-1 ELISA Kit, GPX2 ELISA Kit, gpx1b ELISA Kit, gpx1.S ELISA Kit

Pathways: Thyroid Hormone Synthesis, Sensory Perception of Sound, Skeletal Muscle Fiber Development, Cell RedoxHomeostasis, Negative Regulation of intrinsic apoptotic Signaling, SARS-CoV-2 Protein Interactome