PDK1 ELISA Kit

Catalog No. ABIN5519683

Product Details PDK1 ELISA Kit

Target: PDK1 (Pyruvate Dehydrogenase Kinase 1 (PDK1))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.313 ng/mL - 20 ng/mL

Minimum Detection Limit: 0.313 ng/mL

Application: ELISA

Purpose: For quantitative detection of PDK1 in serum, plasma, tissue homogenates.

Sample Type: Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of PDK1. No significant cross-reactivity or interference between PDK1 and analogues was observed. Note: Limited by current skills and knowledge, it is difficult for us to complete the cross-reactivity detection between PDK1 and all the analogues, therefore, cross reaction may still exist.

Sensitivity: 0.188 ng/mL

Components: - ELISA Microplate
- Lyophilized Standard
- Sample / Standard Dilution Buffer
- Biotin-labeled Antibody (Concentrated)
- Antibody Dilution Buffer
- HRP-Streptavidin Conjugate(SABC)
- SABC Dilution Buffer
- TMB Substrate
- Stop Solution
- Wash Buffer (25X)
- Plate Sealer
- Product Description

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Sandwich ELISA, Double Antibody

Sample Volume: 100 μL

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment:

1. For unopened kit: All the reagents should be kept according to the labels on vials. The Reference Standard and the 96-well stripe plate should be stored at -20 °C upon receipt while the other reagents should be stored at 4 °C.
2. For used kit: When the kit is used, the remaining reagents need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and zip-seal the foil pouch.

Expiry Date: 6 months

Target Details for PDK1

Target: PDK1 (Pyruvate Dehydrogenase Kinase 1 (PDK1))

Alternative Name: Pyruvate Dehydrogenase Kinase Isozyme 1 (PDK1) (PDK1 Products)

Synonyms: ATP5A ELISA Kit, B830012B01 ELISA Kit, D530020C15Rik ELISA Kit, si:zfos-979f1.3 ELISA Kit, 3-phosphoinositide dependent protein kinase-1 ELISA Kit, pyruvate dehydrogenase kinase 1 ELISA Kit, ATP synthase, H+ transporting, mitochondrial Fo complex subunit C2 (subunit 9) ELISA Kit, pyruvate dehydrogenase kinase, isoenzyme 1 ELISA Kit, pyruvate dehydrogenase kinase, isozyme 1 ELISA Kit, pyruvate dehydrogenase kinase 1 L homeolog ELISA Kit, Pdpk1 ELISA Kit, PDK1 ELISA Kit, ATP5G2 ELISA Kit, Pdk1 ELISA Kit, pdk1 ELISA Kit, pdk1.L ELISA Kit

Background: PDK1/PDPK1/[Pyruvate dehydrogenase [lipoamide]] kinase isozyme 1, mitochondrial/mitochondrial pyruvate dehydrogenase, lipoamide, kinase isoenzyme 1/Pyruvate dehydrogenase kinase isoform 1/pyruvate dehydrogenase kinase, isoenzyme 1/pyruvate dehydrogenase k

UniProt: O15530

Pathways: PI3K-Akt Signaling, RTK Signaling, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Warburg Effect