CD73 ELISA Kit

Catalog No. ABIN5519695

Product Details CD73 ELISA Kit

Target: CD73 (NT5E) (5'-Nucleotidase, Ecto (CD73) (NT5E))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.313 ng/mL - 20 ng/mL

Minimum Detection Limit: 0.313 ng/mL

Application: ELISA

Purpose: For quantitative detection of 5-NT in serum, plasma, tissue homogenates.

Sample Type: Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of 5-NT. No significant cross-reactivity or interference between 5-NT and analogues was observed. Note: Limited by current skills and knowledge, it is difficult for us to complete the cross-reactivity detection between 5-NT and all the analogues, therefore, cross reaction may still exist.

Sensitivity: 0.188 ng/mL

Components: - ELISA Microplate
- Lyophilized Standard
- Sample / Standard Dilution Buffer
- Biotin-labeled Antibody (Concentrated)
- Antibody Dilution Buffer
- HRP-Streptavidin Conjugate(SABC)
- SABC Dilution Buffer
- TMB Substrate
- Stop Solution
- Wash Buffer (25X)
- Plate Sealer
- Product Description

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Sandwich ELISA, Double Antibody

Sample Volume: 100 μL

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment:

1. For unopened kit: All the reagents should be kept according to the labels on vials. The Reference Standard and the 96-well stripe plate should be stored at -20 °C upon receipt while the other reagents should be stored at 4 °C.
2. For used kit: When the kit is used, the remaining reagents need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and zip-seal the foil pouch.

Expiry Date: 6 months

References for CD73 ELISA Kit (ABIN5519695)

Merz, Pereira, Schürch, Schefold, Jakob, Takala, Djafarzadeh: "Mitochondrial function of immune cells in septic shock: A prospective observational cohort study." in: PLoS ONE, Vol. 12, Issue 6, pp. e0178946, (2017) (PubMed).

Target Details for CD73

Target: CD73 (NT5E) (5'-Nucleotidase, Ecto (CD73) (NT5E))

Alternative Name: 5-Nucleotidase (NT5E Products)

Synonyms: CALJA ELISA Kit, CD73 ELISA Kit, E5NT ELISA Kit, NT ELISA Kit, NT5 ELISA Kit, NTE ELISA Kit, eN ELISA Kit, eNT ELISA Kit, zgc:63784 ELISA Kit, NT5E ELISA Kit, MGC114869 ELISA Kit, PSPTO2277 ELISA Kit, 2210401F01Rik ELISA Kit, AI447961 ELISA Kit, Nt5 ELISA Kit, 5'-nucleotidase ecto ELISA Kit, 5'-nucleotidase, ecto (CD73) ELISA Kit, 5'-nucleotidase ELISA Kit, 5' nucleotidase, ecto ELISA Kit, NT5E ELISA Kit, nt5e ELISA Kit, nt5e.S ELISA Kit, PSPTO_2277 ELISA Kit, CBU_1233 ELISA Kit, MCA1322 ELISA Kit, Nt5e ELISA Kit

Background: 5-NT/NT5E/5'-NT/CD73/E5NT/eNT/NTE/eN/NT/NT5/5' nucleotidase(CD73)/CD73 antigen/Ecto-5'-nucleotidase/5'-nucleotidase/Purine 5-Prime-Nucleotidase

Pathways: Synaptic Membrane, Ribonucleoside Biosynthetic Process