CYP27B1 ELISA Kit

Catalog No. ABIN5523794

Product Details CYP27B1 ELISA Kit

Target: CYP27B1 (Cytochrome P450, Family 27, Subfamily B, Polypeptide 1 (CYP27B1))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.781 ng/mL - 50 ng/mL

Minimum Detection Limit: 0.781 ng/mL

Application: ELISA

Purpose: For quantitative detection of CYP27B1 in serum, plasma, tissue homogenates.

Sample Type: Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of CYP27B1. No significant cross- reactivity or interference between CYP27B1 and analogues was observed. Note: Limited by current skills and knowledge, it is difficult for us to complete the cross-reactivity detection between CYP27B1 and all the analogues, therefore, cross reaction may still exist.

Sensitivity: 0.469 ng/mL

Components: - ELISA Microplate
- Lyophilized Standard
- Sample / Standard Dilution Buffer
- Biotin-labeled Antibody (Concentrated)
- Antibody Dilution Buffer
- HRP-Streptavidin Conjugate(SABC)
- SABC Dilution Buffer
- TMB Substrate
- Stop Solution
- Wash Buffer (25X)
- Plate Sealer
- Product Description

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Sandwich ELISA, Double Antibody

Sample Volume: 100 μL

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment:

1. For unopened kit: All the reagents should be kept according to the labels on vials. The Reference Standard and the 96-well stripe plate should be stored at -20 °C upon receipt while the other reagents should be stored at 4 °C.
2. For used kit: When the kit is used, the remaining reagents need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and zip-seal the foil pouch.

Expiry Date: 6 months

Target Details for CYP27B1

Target: CYP27B1 (Cytochrome P450, Family 27, Subfamily B, Polypeptide 1 (CYP27B1))

Alternative Name: CYP27B1 (CYP27B1 Products)

Synonyms: cp2b ELISA Kit, cyp1 ELISA Kit, cyp1alpha ELISA Kit, cyp27b ELISA Kit, p450c1 ELISA Kit, pddr ELISA Kit, vdd1 ELISA Kit, vddr ELISA Kit, vddri ELISA Kit, vdr ELISA Kit, CP2B ELISA Kit, CYP1 ELISA Kit, CYP1alpha ELISA Kit, CYP27B ELISA Kit, P450c1 ELISA Kit, PDDR ELISA Kit, VDD1 ELISA Kit, VDDR ELISA Kit, VDDRI ELISA Kit, VDR ELISA Kit, Cyp40 ELISA Kit, Cp2b ELISA Kit, Cyp1 ELISA Kit, Cyp27b ELISA Kit, Pddr ELISA Kit, Vdd1 ELISA Kit, Vddr ELISA Kit, VddrI ELISA Kit, Vdr ELISA Kit, cytochrome P450 family 27 subfamily B member 1 ELISA Kit, cytochrome P450, family 27, subfamily B, polypeptide 1 ELISA Kit, cytochrome P450, family 27, subfamily b, polypeptide 1 ELISA Kit, cyp27b1 ELISA Kit, CYP27B1 ELISA Kit, Cyp27b1 ELISA Kit

Background: CYP27B1/VD3 1A hydroxylase/Cytochrome P450VD1-alpha/Cytochrome P450C1 alpha/Cytochrome P450 subfamily XXVIIB polypeptide 1/Calcidiol 1-monooxygenase/Cytochrome p450 27B1/25-OHD-1 alpha-hydroxylase/25-hydroxyvitamin D(3) 1-alpha-hydroxylase

UniProt: O15528

Pathways: Metabolism of Steroid Hormones and Vitamin D