HIF1A ELISA Kit
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- Target See all HIF1A ELISA Kits
- HIF1A (Hypoxia Inducible Factor 1, alpha Subunit (Basic Helix-Loop-Helix Transcription Factor) (HIF1A))
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Reactivity
- Mouse
- Detection Method
- Colorimetric
- Method Type
- Sandwich ELISA
- Detection Range
- 125 pg/mL - 8000 pg/mL
- Minimum Detection Limit
- 125 pg/mL
- Application
- ELISA
- Purpose
- For quantitative detection of Hif1α in serum, plasma, tissue homogenates.
- Sample Type
- Plasma, Serum, Tissue Homogenate
- Analytical Method
- Quantitative
- Specificity
- This assay has high sensitivity and excellent specificity for detection of Hif1α. No significant cross-reactivity or interference between Hif1α and analogues was observed. Note: Limited by current skills and knowledge, it is difficult for us to complete the cross-reactivity detection between Hif1α and all the analogues, therefore, cross reaction may still exist.
- Sensitivity
- 75 pg/mL
- Components
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- ELISA Microplate
- Lyophilized Standard
- Sample / Standard Dilution Buffer
- Biotin-labeled Antibody (Concentrated)
- Antibody Dilution Buffer
- HRP-Streptavidin Conjugate(SABC)
- SABC Dilution Buffer
- TMB Substrate
- Stop Solution
- Wash Buffer (25X)
- Plate Sealer
- Product Description - Featured
- Discover our best selling HIF1A ELISA Kit
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- Discover our top product HIF1A ELISA Kit
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Comment
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Sandwich ELISA, Double Antibody
- Sample Volume
- 100 μL
- Plate
- Pre-coated
- Restrictions
- For Research Use only
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- Storage
- 4 °C
- Storage Comment
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- For unopened kit: All the reagents should be kept according to the labels on vials. The Reference Standard and the 96-well stripe plate should be stored at -20 °C upon receipt while the other reagents should be stored at 4 °C.
- For used kit: When the kit is used, the remaining reagents need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and zip-seal the foil pouch.
- Expiry Date
- 6 months
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HIF-1α stabilization reduces retinal degeneration in a mouse model of retinitis pigmentosa." in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 32, Issue 5, pp. 2438-2451, (2019) (PubMed).
: "
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HIF-1α stabilization reduces retinal degeneration in a mouse model of retinitis pigmentosa." in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 32, Issue 5, pp. 2438-2451, (2019) (PubMed).
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- Target See all HIF1A ELISA Kits
- HIF1A (Hypoxia Inducible Factor 1, alpha Subunit (Basic Helix-Loop-Helix Transcription Factor) (HIF1A))
- Alternative Name
- Hif1alpha (HIF1A Products)
- Synonyms
- hif1a ELISA Kit, AA959795 ELISA Kit, HIF1alpha ELISA Kit, MOP1 ELISA Kit, bHLHe78 ELISA Kit, HIF-1A ELISA Kit, HIF-1alpha ELISA Kit, HIF1 ELISA Kit, HIF1-ALPHA ELISA Kit, PASD8 ELISA Kit, HIF1-alpha ELISA Kit, hif-1a ELISA Kit, hypoxia inducible factor 1 alpha ELISA Kit, hypoxia inducible factor 1, alpha subunit ELISA Kit, hypoxia inducible factor 1 alpha subunit ELISA Kit, HIF (Hypoxia Inducible Factor) homologa ELISA Kit, hif-1a ELISA Kit, Hif1a ELISA Kit, HIF1A ELISA Kit, hif1a ELISA Kit, hif-1 ELISA Kit
- Background
- Hif1α/HIF-1α/HIF1A/ARNT-interacting protein/Basic-helix-loop-helix-PAS protein MOP1/BHLHE78/Class E basic helix-loop-helix protein 78/HIF-1-alpha/HIF1-alpha/hypoxia-inducible factor 1-alpha/Member of PAS protein 1/member of PAS superfamily 1/PAS domain-co
- UniProt
- Q61221
- Pathways
- Positive Regulation of Peptide Hormone Secretion, Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Cellular Response to Molecule of Bacterial Origin, Carbohydrate Homeostasis, Transition Metal Ion Homeostasis, Tube Formation, Regulation of Carbohydrate Metabolic Process, Signaling Events mediated by VEGFR1 and VEGFR2, VEGFR1 Specific Signals, Warburg Effect
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