iST Sample Preparation Kit

Details for Product No. ABIN5542716
Mass Spectrometry (MS), Sample Preparation (Prep)
Purpose iST Kits offer all-in-one and simple-to-use solutions for reproducible preparation from any starting material to clean peptide mixtures in just 1 hour for LC-MS measurements.
Sample Type Urine, Plasma, Cell Samples, Serum, Tissue Samples
Specificity - single-step lysis, denaturation, alkylation and reduction
- dual clean-up to remove hydrophilic & hydrophobic contaminants
- compatible with standard lab equipment
- results in ready-to-measure peptides
- works with less than 20 μg protein input
- ultrafast sample preparation workflow (total time <2.5 hours)
- more total & exclusive peptide and protein identifications compared to alternative workflows
Characteristics All-in-one for any starting material
- Simple-to-use, sensitive and fast
- Reproducible, reliable and robust
- Only standard lab equipment needed
Components The kit contains all you need to perform a complete sample preparation. It includes all chemicals to denature, reduce and alkylate proteins as well as the enzymes to perform a tryptic digest and a final clean up.
Material not included Common lab equipment is required for the sample preparation.
Application Notes - proteome discovery
- validation of biosimilars
- immunoprecipitations
- body fluid analysis (blood, CSF, serum, urine, and more)

Sample preparation is one of the essential steps of bottom-up proteomics. The sample preparation kit is designed to assist you achieving best results with few sample preparation steps and little hands-on time.

Assay Time 4 - 5 h
Assay Precision high reproducibility (R2 >0.9)
Restrictions For Research Use only
Storage RT,-20 °C
Supplier Images
 image for iST Sample Preparation Kit (ABIN5542716) iST Sample Preparation Kit
Product cited in: Doll, Kriegmair, Santos, Wierer, Coscia, Neil, Porubsky, Geyer, Mund, Nuhn, Mann: "Rapid proteomic analysis for solid tumors reveals LSD1 as a drug target in an end-stage cancer patient." in: Molecular oncology, 2018 (PubMed).

Karg, Smets, Ryan, Forné, Qin, Mulholland, Kalideris, Imhof, Bultmann, Leonhardt: "Ubiquitome Analysis Reveals PCNA-Associated Factor 15 (PAF15) as a Specific Ubiquitination Target of UHRF1 in Embryonic Stem Cells." in: Journal of molecular biology, 2017 (PubMed).