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Adrenomedullin 2 ELISA Kit

ADM2 Reactivity: Human Colorimetric Competition ELISA 246.9 pg/mL - 20000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Catalog No. ABIN5651499
  • Target See all Adrenomedullin 2 (ADM2) ELISA Kits
    Adrenomedullin 2 (ADM2)
    Reactivity
    • 3
    • 1
    Human
    Detection Method
    Colorimetric
    Method Type
    Competition ELISA
    Detection Range
    246.9 pg/mL - 20000 pg/mL
    Minimum Detection Limit
    246.9 pg/mL
    Application
    ELISA
    Sample Type
    Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
    Analytical Method
    Quantitative
    Specificity
    This assay has high sensitivity and excellent specificity for detection of Adrenomedullin 2 (ADM2). No significant cross-reactivity or interference between Adrenomedullin 2 (ADM2) and analogues was observed.
    Sensitivity
    91.5 pg/mL
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  • Comment

    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

    Assay Time
    2 h
    Plate
    Pre-coated
    Protocol
    This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Adrenomedullin 2 (ADM2) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Adrenomedullin 2 (ADM2) and unlabeled Adrenomedullin 2 (ADM2) (Standards or samples) with the pre-coated antibody specific to Adrenomedullin 2 (ADM2). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Adrenomedullin 2 (ADM2) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Adrenomedullin 2 (ADM2) in the sample.
    Assay Precision
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Adrenomedullin 2 (ADM2) were tested 20 times on one plate, respectively
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Adrenomedullin 2 (ADM2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%
    Restrictions
    For Research Use only
  • Handling Advice
    The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
    Storage
    4 °C,-20 °C
    Storage Comment
    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
    Expiry Date
    4-8 months
  • Target See all Adrenomedullin 2 (ADM2) ELISA Kits
    Adrenomedullin 2 (ADM2)
    Alternative Name
    Adrenomedullin 2 (ADM2 Products)
    Synonyms
    adm2 ELISA Kit, imdn ELISA Kit, si:dkey-22l11.3 ELISA Kit, ADM2 ELISA Kit, am2 ELISA Kit, AM2 ELISA Kit, dJ579N16.4 ELISA Kit, Am2 ELISA Kit, IMD ELISA Kit, Imdn ELISA Kit, Imd ELISA Kit, adrenomedullin 2a ELISA Kit, adrenomedullin 2 ELISA Kit, adm2a ELISA Kit, ADM2 ELISA Kit, adm2 ELISA Kit, Adm2 ELISA Kit
    Background

    Gene Name: Adrenomedullin 2

    Gene Aliases: AM2, IMDL, IMDS, Intermedin

    Gene ID
    79924
    UniProt
    Q7Z4H4
    Pathways
    Hormone Activity, Feeding Behaviour
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