Intestinal Alkaline Phosphatase ELISA Kit

Catalog No. ABIN5651582

Product Details Intestinal Alkaline Phosphatase ELISA Kit

Target: Intestinal Alkaline Phosphatase (ALPI)

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 1.56 ng/mL - 100 ng/mL

Minimum Detection Limit: 1.56 ng/mL

Application: ELISA

Sample Type: Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Alkaline Phosphatase, Intestinal (ALPI). No significant cross-reactivity or interference between Alkaline Phosphatase, Intestinal (ALPI) and analogues was observed.

Sensitivity: 0.65 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Alkaline Phosphatase, Intestinal (ALPI). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Alkaline Phosphatase, Intestinal (ALPI). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Alkaline Phosphatase, Intestinal (ALPI), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Alkaline Phosphatase, Intestinal (ALPI) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Alkaline Phosphatase, Intestinal (ALPI) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Alkaline Phosphatase, Intestinal (ALPI) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for Intestinal Alkaline Phosphatase

Target: Intestinal Alkaline Phosphatase (ALPI)

Alternative Name: Alkaline Phosphatase, Intestinal (ALPI Products)

Synonyms: IAP ELISA Kit, Alp1 ELISA Kit, S51097 ELISA Kit, 2010001C14Rik ELISA Kit, Akp6 ELISA Kit, gIAP ELISA Kit, alpi ELISA Kit, im:7149588 ELISA Kit, si:ch211-262h13.1 ELISA Kit, wu:fa95f04 ELISA Kit, zgc:110794 ELISA Kit, ALPI ELISA Kit, Alpi ELISA Kit, alkaline phosphatase, intestinal ELISA Kit, alkaline phosphatase, intestinal, tandem duplicate 1 ELISA Kit, intestinal-type alkaline phosphatase ELISA Kit, ALPI ELISA Kit, Alpi ELISA Kit, alpi.1 ELISA Kit, LOC100724954 ELISA Kit

Background:

Gene Name: Alkaline Phosphatase, Intestinal

Gene Aliases: CIP, IAP, Calf Intestinal Alkaline Phosphatase

Gene ID: 248

UniProt: P09923