ACE2 ELISA Kit

Catalog No. ABIN5651788

Product Details ACE2 ELISA Kit

Target: ACE2 (Angiotensin I Converting Enzyme 2 (ACE2))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 12.5 pg/mL - 800 pg/mL

Minimum Detection Limit: 12.5 pg/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Angiotensin I Converting Enzyme 2 (ACE2). No significant cross-reactivity or interference between Angiotensin I Converting Enzyme 2 (ACE2) and analogues was observed.

Sensitivity: 5.5 pg/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Angiotensin I Converting Enzyme 2 (ACE2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Angiotensin I Converting Enzyme 2 (ACE2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Angiotensin I Converting Enzyme 2 (ACE2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Angiotensin I Converting Enzyme 2 (ACE2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Angiotensin I Converting Enzyme 2 (ACE2) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Angiotensin I Converting Enzyme 2 (ACE2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for ACE2

Target: ACE2 (Angiotensin I Converting Enzyme 2 (ACE2))

Alternative Name: Angiotensin I Converting Enzyme 2 (ACE2 Products)

Synonyms: ACE1 ELISA Kit, CD143 ELISA Kit, DCP ELISA Kit, DCP1 ELISA Kit, ICH ELISA Kit, MVCD3 ELISA Kit, ACEH ELISA Kit, 2010305L05Rik ELISA Kit, zgc:92514 ELISA Kit, angiotensin I converting enzyme ELISA Kit, angiotensin I converting enzyme 2 ELISA Kit, angiotensin I converting enzyme (peptidyl-dipeptidase A) 2 ELISA Kit, ACE ELISA Kit, ACE2 ELISA Kit, Ace2 ELISA Kit, ace2 ELISA Kit

Background:

Gene Name: Angiotensin I Converting Enzyme 2

Gene Aliases: ACEH, ACEII, ACE-II, Peptidyl-Dipeptidase A, ACE-related carboxypeptidase, Angiotensin-converting enzyme homolog, Metalloprotease MPROT15

Gene ID: 70008

UniProt: Q8R0I0

Pathways: ACE Inhibitor Pathway, Peptide Hormone Metabolism, Regulation of Systemic Arterial Blood Pressure by Hormones, Feeding Behaviour