APAF1 CLIA Kit

Catalog No. ABIN5651961

Product Details APAF1 CLIA Kit

Target: APAF1 (Apoptotic Peptidase Activating Factor 1 (APAF1))

Reactivity: Human

Detection Method: Chemiluminescent

Method Type: Sandwich ELISA

Detection Range: 156.25 pg/mL - 10000 pg/mL

Minimum Detection Limit: 156.25 pg/mL

Application: ELISA

Sample Type: Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Apoptotic Peptidase Activating Factor 1 (APAF1). No significant cross-reactivity or interference between Apoptotic Peptidase Activating Factor 1 (APAF1) and analogues was observed.

Sensitivity: 6.27 pg/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 2 - 3 h

Plate: Pre-coated

Protocol: The microplate provided in this kit has been pre-coated with an antibody specific to Apoptotic Peptidase Activating Factor 1 (APAF1). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Apoptotic Peptidase Activating Factor 1 (APAF1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Apoptotic Peptidase Activating Factor 1 (APAF1) level in the sample or standard.,

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Apoptotic Peptidase Activating Factor 1 (APAF1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Apoptotic Peptidase Activating Factor 1 (APAF1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details

Target: APAF1 (Apoptotic Peptidase Activating Factor 1 (APAF1))

Alternative Name: Apoptotic Peptidase Activating Factor 1 (APAF1 Products)

Synonyms: APAF1 CLIA Kit, ARK CLIA Kit, Apaf-1 CLIA Kit, Apaf1 CLIA Kit, CG6829 CLIA Kit, D-Apaf-1 CLIA Kit, DARK CLIA Kit, Dapaf-1 CLIA Kit, Dapaf-1/HAC-1 CLIA Kit, Dark CLIA Kit, Dark/Apaf-I CLIA Kit, Dark/Dapaf-1/HAC1 CLIA Kit, Dark/Hac-1/dApaf-1 CLIA Kit, Dark/Hac-1/dApaf1 CLIA Kit, Dmel\\CG6829 CLIA Kit, Hac-1 CLIA Kit, Hac-1/Dark CLIA Kit, Hac1 CLIA Kit, T1 CLIA Kit, anon-53Fa CLIA Kit, apaf-1 CLIA Kit, apaf1 CLIA Kit, arc CLIA Kit, ark CLIA Kit, dAPAF-1 CLIA Kit, dApaf-1 CLIA Kit, dApaf-1/DARK/HAC-1 CLIA Kit, dApaf1 CLIA Kit, dArk CLIA Kit, dapaf CLIA Kit, dapaf-1 CLIA Kit, dapaf-1L CLIA Kit, dapaf-1S CLIA Kit, dark CLIA Kit, dark/dapaf-1/hac-1 CLIA Kit, dark/hac-1/dapaf-1 CLIA Kit, hac-1 CLIA Kit, hac1 CLIA Kit, l(2)SH0173 CLIA Kit, APAF-1 CLIA Kit, CED4 CLIA Kit, 6230400I06Rik CLIA Kit, Apaf1l CLIA Kit, fog CLIA Kit, mKIAA0413 CLIA Kit, zgc:136543 CLIA Kit, Death-associated APAF1-related killer CLIA Kit, apoptotic peptidase activating factor 1 L homeolog CLIA Kit, apoptotic peptidase activating factor 1 CLIA Kit, Dark CLIA Kit, apaf1.L CLIA Kit, apaf1 CLIA Kit, APAF1 CLIA Kit, Apaf1 CLIA Kit

Background:

Gene Name: Apoptotic Peptidase Activating Factor 1

Gene Aliases: CED4, Apoptotic Protease Activating Factor

Gene ID: 317

UniProt: O14727

Pathways: p53 Signaling, Apoptosis, Caspase Cascade in Apoptosis, Tube Formation, Positive Regulation of Endopeptidase Activity