ATP7B ELISA Kit

Catalog No. ABIN5652087

Product Details ATP7B ELISA Kit

Target: ATP7B (ATPase, Cu++ Transporting, beta Polypeptide (ATP7B))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.156 ng/mL - 10 ng/mL

Minimum Detection Limit: 0.156 ng/mL

Application: ELISA

Sample Type: Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of ATPase, Cu++ Transporting Beta Polypeptide (ATP7b). No significant cross-reactivity or interference between ATPase, Cu++ Transporting Beta Polypeptide (ATP7b) and analogues was observed.

Sensitivity: 0.056 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to ATPase, Cu++ Transporting Beta Polypeptide (ATP7b). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to ATPase, Cu++ Transporting Beta Polypeptide (ATP7b). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain ATPase, Cu++ Transporting Beta Polypeptide (ATP7b), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of ATPase, Cu++ Transporting Beta Polypeptide (ATP7b) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level ATPase, Cu++ Transporting Beta Polypeptide (ATP7b) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level ATPase, Cu++ Transporting Beta Polypeptide (ATP7b) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for ATP7B

Target: ATP7B (ATPase, Cu++ Transporting, beta Polypeptide (ATP7B))

Alternative Name: ATPase, Cu++ Transporting Beta Polypeptide (ATP7B Products)

Synonyms: PWD ELISA Kit, WC1 ELISA Kit, WD ELISA Kit, WND ELISA Kit, Atp7a ELISA Kit, tx ELISA Kit, Hts ELISA Kit, PINA ELISA Kit, Wd ELISA Kit, ATP7B ELISA Kit, ATPase copper transporting beta ELISA Kit, ATPase, Cu++ transporting, beta polypeptide ELISA Kit, ATPase, Cu++ transporting, beta polypeptide (Wilson disease) ELISA Kit, copper-transporting ATPase 2 ELISA Kit, ATP7B ELISA Kit, Atp7b ELISA Kit, atp7b ELISA Kit, LOC592143 ELISA Kit

Background:

Gene Name: ATPase, Cu++ Transporting Beta Polypeptide

Gene Aliases: PWD, WC1, WD, WND, Wilson Disease Protein, Copper pump 2, Wilson disease-associated protein

Gene ID: 540

UniProt: P35670

Pathways: Transition Metal Ion Homeostasis, Ribonucleoside Biosynthetic Process