ATP6AP2 ELISA Kit

Catalog No. ABIN5652089

Product Details ATP6AP2 ELISA Kit

Target: ATP6AP2 (ATPase, H+ Transporting, Lysosomal Accessory Protein 2 (ATP6AP2))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.312 ng/mL - 20 ng/mL

Minimum Detection Limit: 0.312 ng/mL

Application: ELISA

Sample Type: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of ATPase, H+ Transporting, Lysosomal Accessory Protein 2 (ATP6AP2). No significant cross-reactivity or interference between ATPase, H+ Transporting, Lysosomal Accessory Protein 2 (ATP6AP2) and analogues was observed.

Sensitivity: 0.115 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to ATPase, H+ Transporting, Lysosomal Accessory Protein 2 (ATP6AP2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to ATPase, H+ Transporting, Lysosomal Accessory Protein 2 (ATP6AP2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain ATPase, H+ Transporting, Lysosomal Accessory Protein 2 (ATP6AP2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of ATPase, H+ Transporting, Lysosomal Accessory Protein 2 (ATP6AP2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level ATPase, H+ Transporting, Lysosomal Accessory Protein 2 (ATP6AP2) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level ATPase, H+ Transporting, Lysosomal Accessory Protein 2 (ATP6AP2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for ATP6AP2

Target: ATP6AP2 (ATPase, H+ Transporting, Lysosomal Accessory Protein 2 (ATP6AP2))

Alternative Name: ATPase, H+ Transporting, Lysosomal Accessory Protein 2 (ATP6AP2 Products)

Synonyms: APT6M8-9 ELISA Kit, ATP6IP2 ELISA Kit, ATP6M8-9 ELISA Kit, ELDF10 ELISA Kit, M8-9 ELISA Kit, MRXE ELISA Kit, MSTP009 ELISA Kit, XMRE ELISA Kit, (P)RR ELISA Kit, 5730403E06Rik ELISA Kit, Atp6ip2 ELISA Kit, tj6 ELISA Kit, wss ELISA Kit, arcl ELISA Kit, j6b7 ELISA Kit, stv1 ELISA Kit, tj6m ELISA Kit, tj6s ELISA Kit, vph1 ELISA Kit, atp6a2 ELISA Kit, atp6n1d ELISA Kit, ATP6AP2 ELISA Kit, DKFZp459G0918 ELISA Kit, atp6ap2 ELISA Kit, wu:fj36c03 ELISA Kit, wu:fq15e04 ELISA Kit, zgc:73194 ELISA Kit, ATPase H+ transporting accessory protein 2 ELISA Kit, ATPase, H+ transporting, lysosomal accessory protein 2 ELISA Kit, ATPase H+ transporting V0 subunit d2 ELISA Kit, ATPase H+ transporting V0 subunit a2 ELISA Kit, ATPase, H+ transporting, lysosomal accessory protein 2 S homeolog ELISA Kit, ATP6AP2 ELISA Kit, Atp6ap2 ELISA Kit, ATP6V0D2 ELISA Kit, atp6v0a2 ELISA Kit, atp6ap2.S ELISA Kit, atp6ap2 ELISA Kit

Background:

Gene Name: ATPase, H+ Transporting, Lysosomal Accessory Protein 2

Gene Aliases: ATP6IP2, CAPER, ELDF10, XMRE, Renin/prorenin receptor, Embryonic liver differentiation factor 10, Vacuolar ATP synthase membrane sector-associated protein M8-9

Gene ID: 10159

UniProt: O75787

Pathways: ACE Inhibitor Pathway, Peptide Hormone Metabolism, Regulation of Systemic Arterial Blood Pressure by Hormones