BMP4 CLIA Kit

Catalog No. ABIN5652275

Product Details BMP4 CLIA Kit

Target: BMP4 (Bone Morphogenetic Protein 4 (BMP4))

Reactivity: Mouse

Detection Method: Chemiluminescent

Method Type: Sandwich ELISA

Detection Range: 156.25 pg/mL - 10000 pg/mL

Minimum Detection Limit: 156.25 pg/mL

Application: ELISA

Sample Type: Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Bone Morphogenetic Protein 4 (BMP4). No significant cross-reactivity or interference between Bone Morphogenetic Protein 4 (BMP4) and analogues was observed.

Sensitivity: 4.86 pg/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 2 - 3 h

Plate: Pre-coated

Protocol: The microplate provided in this kit has been pre-coated with an antibody specific to Bone Morphogenetic Protein 4 (BMP4). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Bone Morphogenetic Protein 4 (BMP4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Bone Morphogenetic Protein 4 (BMP4) level in the sample or standard.,

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Bone Morphogenetic Protein 4 (BMP4) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Bone Morphogenetic Protein 4 (BMP4) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details

Target: BMP4 (Bone Morphogenetic Protein 4 (BMP4))

Alternative Name: Bone Morphogenetic Protein 4 (BMP4 Products)

Synonyms: BMP2B CLIA Kit, BMP2B1 CLIA Kit, MCOPS6 CLIA Kit, OFC11 CLIA Kit, ZYME CLIA Kit, Bmp-4 CLIA Kit, Bmp2b CLIA Kit, Bmp2b-1 CLIA Kit, Bmp2b1 CLIA Kit, BOMPR4A CLIA Kit, bmp-4 CLIA Kit, zbmp-4 CLIA Kit, zgc:100779 CLIA Kit, BMP-4 CLIA Kit, XBMP-4 CLIA Kit, bmp2b CLIA Kit, bmp2b1 CLIA Kit, bmp4 CLIA Kit, ofc11 CLIA Kit, xbmp4 CLIA Kit, zyme CLIA Kit, BMP4 CLIA Kit, bone morphogenetic protein 4 CLIA Kit, bone morphogenetic protein 4 L homeolog CLIA Kit, bone morphogenetic protein 4 S homeolog CLIA Kit, BMP4 CLIA Kit, Bmp4 CLIA Kit, bmp4 CLIA Kit, bmp4.L CLIA Kit, bmp4.S CLIA Kit

Background:

Gene Name: Bone Morphogenetic Protein 4

Gene Aliases: BMP2B, BMP2-B, BMP2B1, ZYME, DVR4, Bone morphogenetic protein 2B

Pathways: Steroid Hormone Mediated Signaling Pathway, Regulation of Muscle Cell Differentiation, Tube Formation, Skeletal Muscle Fiber Development