CRP CLIA Kit

Catalog No. ABIN5652374

Product Details CRP CLIA Kit

Target: CRP (C-Reactive Protein (CRP))

Reactivity: Rat

Detection Method: Chemiluminescent

Method Type: Sandwich ELISA

Detection Range: 0.39 ng/mL - 100 ng/mL

Minimum Detection Limit: 0.39 ng/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of C Reactive Protein (CRP). No significant cross-reactivity or interference between C Reactive Protein (CRP) and analogues was observed.

Sensitivity: 0.05 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 2 - 3 h

Plate: Pre-coated

Protocol: The microplate provided in this kit has been pre-coated with an antibody specific to C Reactive Protein (CRP). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to C Reactive Protein (CRP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the C Reactive Protein (CRP) level in the sample or standard.,

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level C Reactive Protein (CRP) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level C Reactive Protein (CRP) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details

Target: CRP (C-Reactive Protein (CRP))

Alternative Name: C Reactive Protein (CRP Products)

Synonyms: PTX1 CLIA Kit, crp CLIA Kit, AI255847 CLIA Kit, Aa1249 CLIA Kit, Ab1-341 CLIA Kit, Ab2-196 CLIA Kit, Ac1-114 CLIA Kit, Ac1262 CLIA Kit, Ac2-069 CLIA Kit, Ba2-693 CLIA Kit, APCS CLIA Kit, 0610010I23Rik CLIA Kit, AW743261 CLIA Kit, C77570 CLIA Kit, CRP2 CLIA Kit, CRP4 CLIA Kit, Crp CLIA Kit, ESP1 CLIA Kit, Hlp CLIA Kit, CRP5.1 CLIA Kit, zgc:152809 CLIA Kit, C-reactive protein CLIA Kit, C-reactive protein, pentraxin-related CLIA Kit, c-reactive protein, pentraxin-related CLIA Kit, cysteine rich protein 2 CLIA Kit, CRP CLIA Kit, crp CLIA Kit, Crp CLIA Kit, Crip2 CLIA Kit, LOC776376 CLIA Kit

Background:

Gene Name: C Reactive Protein

Gene Aliases: C-RP, PTX1, Pentraxin-Related, Pentraxin 1

Gene ID: 25419

UniProt: P48199

Pathways: Carbohydrate Homeostasis