CAN ELISA Kit

Catalog No. ABIN5652470

Product Details CAN ELISA Kit

Target: CAN (Calcineurin (CAN))

Reactivity: Rat

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.156 ng/mL - 10 ng/mL

Minimum Detection Limit: 0.156 ng/mL

Application: ELISA

Sample Type: Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Calcineurin (CaN). No significant cross-reactivity or interference between Calcineurin (CaN) and analogues was observed.

Sensitivity: 0.064 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Calcineurin (CaN). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Calcineurin (CaN). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Calcineurin (CaN), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Calcineurin (CaN) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Calcineurin (CaN) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Calcineurin (CaN) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for CAN

Target: CAN (Calcineurin (CAN))

Alternative Name: Calcineurin (CAN Products)

Synonyms: CALN ELISA Kit, CALNA ELISA Kit, CALNA1 ELISA Kit, CCN1 ELISA Kit, CNA1 ELISA Kit, PPP2B ELISA Kit, CAN A1/PP2B ELISA Kit, CG1455 ELISA Kit, Can ELISA Kit, Cana1 ELISA Kit, CnA ELISA Kit, CnnA21EF ELISA Kit, D14 ELISA Kit, DmCanA1-100B ELISA Kit, Dmel\\CG1455 ELISA Kit, PP2B ELISA Kit, PP2B 21EF ELISA Kit, PpD14 ELISA Kit, canA ELISA Kit, pMY4 ELISA Kit, 2900074D19Rik ELISA Kit, AI841391 ELISA Kit, AW413465 ELISA Kit, CN ELISA Kit, Caln ELISA Kit, Calna ELISA Kit, Calna1 ELISA Kit, protein phosphatase 3 catalytic subunit alpha ELISA Kit, Calcineurin A1 ELISA Kit, protein phosphatase 3, catalytic subunit, alpha isoform ELISA Kit, PPP3CA ELISA Kit, CanA1 ELISA Kit, Ppp3ca ELISA Kit

Background:

Gene Name: Calcineurin

Gene Aliases: PPP3CA, PPP3-CA, CCN1, CNA1, CALN, CALNA1, PP2B, PP2-B, Protein Phosphatase 3(formerly 2B),Catalytic Subunit,Alpha Isoformprotein Phosphatase 2B, Calcineurin A Alpha