CACNA1C ELISA Kit

Catalog No. ABIN5652483

Product Details CACNA1C ELISA Kit

Target: CACNA1C (Calcium Channel, Voltage-Dependent, L Type, alpha 1C Subunit (CACNA1C))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.312 ng/mL - 20 ng/mL

Minimum Detection Limit: 0.312 ng/mL

Application: ELISA

Sample Type: Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Calcium Channel, Voltage Dependent, L-Type, Alpha 1C Subunit (CACNa1C). No significant cross-reactivity or interference between Calcium Channel, Voltage Dependent, L-Type, Alpha 1C Subunit (CACNa1C) and analogues was observed.

Sensitivity: 0.115 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Calcium Channel, Voltage Dependent, L-Type, Alpha 1C Subunit (CACNa1C). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Calcium Channel, Voltage Dependent, L-Type, Alpha 1C Subunit (CACNa1C). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Calcium Channel, Voltage Dependent, L-Type, Alpha 1C Subunit (CACNa1C), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Calcium Channel, Voltage Dependent, L-Type, Alpha 1C Subunit (CACNa1C) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Calcium Channel, Voltage Dependent, L-Type, Alpha 1C Subunit (CACNa1C) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Calcium Channel, Voltage Dependent, L-Type, Alpha 1C Subunit (CACNa1C) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for CACNA1C

Target: CACNA1C (Calcium Channel, Voltage-Dependent, L Type, alpha 1C Subunit (CACNA1C))

Alternative Name: Calcium Channel, Voltage Dependent, L-Type, Alpha 1C Subunit (CACNA1C Products)

Synonyms: CACH2 ELISA Kit, CACN2 ELISA Kit, CACNL1A1 ELISA Kit, CCHL1A1 ELISA Kit, CaV1.2 ELISA Kit, LQT8 ELISA Kit, TS ELISA Kit, Cav1.2 ELISA Kit, Cchl1a1 ELISA Kit, MBC ELISA Kit, MELC-CC ELISA Kit, RATIVS302 ELISA Kit, isl ELISA Kit, C-LTCC ELISA Kit, CHCACHA1C ELISA Kit, ICA ELISA Kit, cav1.2 ELISA Kit, ca(v)1.2 ELISA Kit, CAV1.2 ELISA Kit, BCR ELISA Kit, rbc ELISA Kit, calcium voltage-gated channel subunit alpha1 C ELISA Kit, calcium channel, voltage-dependent, L type, alpha 1C subunit ELISA Kit, voltage-dependent L-type calcium channel subunit alpha-1C ELISA Kit, calcium channel, voltage-dependent, L type, alpha 1C subunit S homeolog ELISA Kit, Red blood cell count ELISA Kit, RNA, 7SL, cytoplasmic 263, pseudogene ELISA Kit, CACNA1C ELISA Kit, Cacna1c ELISA Kit, cacna1c ELISA Kit, LOC100442085 ELISA Kit, cacna1c.S ELISA Kit, RBC ELISA Kit, RN7SL263P ELISA Kit

Background:

Gene Name: Calcium Channel, Voltage Dependent, L-Type, Alpha 1C Subunit

Gene Aliases: CACH2, CACN2, CACNL1A1, CCHL1A1, CaV1.2, TS, Voltage-gated calcium channel subunit alpha Cav1.2, Calcium channel, L type, alpha-1 polypeptide, isoform 1, cardiac muscle

Gene ID: 12288

UniProt: Q01815

Pathways: Hormone Transport, Carbohydrate Homeostasis