DFFB ELISA Kit

Catalog No. ABIN5652668

Product Details DFFB ELISA Kit

Target: DFFB (DNA Fragmentation Factor, 40kDa, beta Polypeptide (Caspase-Activated DNase) (DFFB))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.156 ng/mL - 10 ng/mL

Minimum Detection Limit: 0.156 ng/mL

Application: ELISA

Sample Type: Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Caspase Activated DNase (CAD). No significant cross-reactivity or interference between Caspase Activated DNase (CAD) and analogues was observed.

Sensitivity: 0.052 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Caspase Activated DNase (CAD). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Caspase Activated DNase (CAD). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Caspase Activated DNase (CAD), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Caspase Activated DNase (CAD) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Caspase Activated DNase (CAD) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Caspase Activated DNase (CAD) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for DFFB

Target: DFFB (DNA Fragmentation Factor, 40kDa, beta Polypeptide (Caspase-Activated DNase) (DFFB))

Alternative Name: Caspase Activated DNase (DFFB Products)

Synonyms: CAD ELISA Kit, CPAN ELISA Kit, DFF-40 ELISA Kit, DFF2 ELISA Kit, DFF40 ELISA Kit, LOC100223514 ELISA Kit, 40kDa ELISA Kit, 5730477D02Rik ELISA Kit, Didff ELISA Kit, Cad ELISA Kit, carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase ELISA Kit, DNA fragmentation factor subunit beta ELISA Kit, DNA fragmentation factor, beta polypeptide (caspase-activated DNase) ELISA Kit, DNA fragmentation factor, beta subunit ELISA Kit, CAD ELISA Kit, DFFB ELISA Kit, dffb ELISA Kit, LOC100223514 ELISA Kit, Dffb ELISA Kit

Background:

Gene Name: Caspase Activated DNase

Gene Aliases: DFF-B, DFFB, CPAN, DFF40, DFF2, DNA Fragmentation Factor 40 kDa Beta Polypeptide, Caspase-activated deoxyribonuclease, Caspase-activated nuclease

Gene ID: 1677

UniProt: O76075

Pathways: Apoptosis, Caspase Cascade in Apoptosis