CECR1 CLIA Kit

Catalog No. ABIN5652670

Product Details CECR1 CLIA Kit

Target: CECR1 (Cat Eye Syndrome Chromosome Region, Candidate 1 (CECR1))

Reactivity: Human

Detection Method: Chemiluminescent

Method Type: Sandwich ELISA

Detection Range: 312.5 pg/mL - 20000 pg/mL

Minimum Detection Limit: 312.5 pg/mL

Application: ELISA

Sample Type: Plasma, Serum

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Cat Eye Syndrome Chromosome Region, Candidate 1 (CECR1). No significant cross-reactivity or interference between Cat Eye Syndrome Chromosome Region, Candidate 1 (CECR1) and analogues was observed.

Sensitivity: 9.58 pg/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 2 - 3 h

Plate: Pre-coated

Protocol: The microplate provided in this kit has been pre-coated with an antibody specific to Cat Eye Syndrome Chromosome Region, Candidate 1 (CECR1). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Cat Eye Syndrome Chromosome Region, Candidate 1 (CECR1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Cat Eye Syndrome Chromosome Region, Candidate 1 (CECR1) level in the sample or standard.,

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cat Eye Syndrome Chromosome Region, Candidate 1 (CECR1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cat Eye Syndrome Chromosome Region, Candidate 1 (CECR1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details

Target: CECR1 (Cat Eye Syndrome Chromosome Region, Candidate 1 (CECR1))

Alternative Name: Cat Eye Syndrome Chromosome Region, Candidate 1 (CECR1 Products)

Synonyms: cecr1 CLIA Kit, fj65e02 CLIA Kit, wu:fj65e02 CLIA Kit, CECR1 CLIA Kit, ADA2 CLIA Kit, ADGF CLIA Kit, IDGFL CLIA Kit, adenosine deaminase 2a CLIA Kit, cat eye syndrome chromosome region, candidate 1 CLIA Kit, adenosine deaminase 2 CLIA Kit, adenosine deaminase 2 L homeolog CLIA Kit, ada2a CLIA Kit, CECR1 CLIA Kit, ADA2 CLIA Kit, ada2.L CLIA Kit

Background:

Gene Name: Cat Eye Syndrome Chromosome Region, Candidate 1

Gene Aliases: IDGFL, ADGF, ADA2, Cat Eye Syndrome Critical Region Protein 1

Gene ID: 51816

UniProt: Q9NZK5

Pathways: Ribonucleoside Biosynthetic Process