Cathepsin D CLIA Kit

Catalog No. ABIN5652698

Product Details Cathepsin D CLIA Kit

Target: Cathepsin D (CTSD)

Reactivity: Mouse

Detection Method: Chemiluminescent

Method Type: Sandwich ELISA

Detection Range: 31.25 pg/mL - 2000 pg/mL

Minimum Detection Limit: 31.25 pg/mL

Application: ELISA

Sample Type: Plasma, Serum

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Cathepsin D (CTSD). No significant cross-reactivity or interference between Cathepsin D (CTSD) and analogues was observed.

Sensitivity: 0.98 pg/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 2 - 3 h

Plate: Pre-coated

Protocol: The microplate provided in this kit has been pre-coated with an antibody specific to Cathepsin D (CTSD). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Cathepsin D (CTSD). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Cathepsin D (CTSD) level in the sample or standard.,

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cathepsin D (CTSD) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cathepsin D (CTSD) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details

Target: Cathepsin D (CTSD)

Alternative Name: Cathepsin D (CTSD Products)

Synonyms: catD CLIA Kit, fb93e11 CLIA Kit, fj17b09 CLIA Kit, wu:fb93e11 CLIA Kit, wu:fj17b09 CLIA Kit, LOC398557 CLIA Kit, Cathd CLIA Kit, CatD CLIA Kit, LOC100136761 CLIA Kit, CTSD CLIA Kit, DKFZp469J0315 CLIA Kit, DDBDRAFT_0205785 CLIA Kit, DDBDRAFT_0215012 CLIA Kit, DDB_0205785 CLIA Kit, DDB_0215012 CLIA Kit, CLN10 CLIA Kit, CPSD CLIA Kit, CD CLIA Kit, cathepsin D CLIA Kit, cathepsin D S homeolog CLIA Kit, lysosomal aspartic protease CLIA Kit, cathepsin D-like CLIA Kit, ctsd CLIA Kit, LOC398557 CLIA Kit, ctsd.S CLIA Kit, CatD CLIA Kit, LOC5567565 CLIA Kit, LOC100136761 CLIA Kit, CTSD CLIA Kit, Cathd CLIA Kit, ctsD CLIA Kit, catd CLIA Kit, Ctsd CLIA Kit, LOC443060 CLIA Kit

Background:

Gene Name: Cathepsin D

Gene Aliases: CPSD, CLN10, Lysosomal Aspartyl Protease, Ceroid-Lipofuscinosis,Neuronal 10

Gene ID: 13033

UniProt: P18242

Pathways: Peptide Hormone Metabolism