CCKAR CLIA Kit

Catalog No. ABIN5652899

Product Details CCKAR CLIA Kit

Target: CCKAR (Cholecystokinin A Receptor (CCKAR))

Reactivity: Rabbit

Detection Method: Chemiluminescent

Method Type: Sandwich ELISA

Detection Range: 312.5 pg/mL - 20000 pg/mL

Minimum Detection Limit: 312.5 pg/mL

Application: ELISA

Sample Type: Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Cholecystokinin A Receptor (CCKAR). No significant cross-reactivity or interference between Cholecystokinin A Receptor (CCKAR) and analogues was observed.

Sensitivity: 11.29 pg/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 2 - 3 h

Plate: Pre-coated

Protocol: The microplate provided in this kit has been pre-coated with an antibody specific to Cholecystokinin A Receptor (CCKAR). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Cholecystokinin A Receptor (CCKAR). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Cholecystokinin A Receptor (CCKAR) level in the sample or standard.,

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cholecystokinin A Receptor (CCKAR) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cholecystokinin A Receptor (CCKAR) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details

Target: CCKAR (Cholecystokinin A Receptor (CCKAR))

Alternative Name: Cholecystokinin A Receptor (CCKAR Products)

Synonyms: CCK-XL CLIA Kit, cck-a CLIA Kit, cck1-r CLIA Kit, cckar-A CLIA Kit, cckbr CLIA Kit, cckra CLIA Kit, CCKAR CLIA Kit, CCK-A CLIA Kit, CCK1-R CLIA Kit, CCKRA CLIA Kit, AW106902 CLIA Kit, Cck-ar CLIA Kit, CCK-AR CLIA Kit, cholecystokinin A receptor CLIA Kit, cholecystokinin A receptor L homeolog CLIA Kit, CCKAR CLIA Kit, cckar.L CLIA Kit, Cckar CLIA Kit

Background:

Gene Name: Cholecystokinin A Receptor

Gene Aliases: CCK-A, CCK1, CCKRA, CCK1R, Cholecystokinin Type 1 Receptor

Pathways: Positive Regulation of Peptide Hormone Secretion, Hormone Transport, Feeding Behaviour