COL2A1 ELISA Kit

Catalog No. ABIN5653112

Product Details COL2A1 ELISA Kit

Target: COL2A1 (Collagen, Type II, alpha 1 (COL2A1))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 31.25 pg/mL - 2000 pg/mL

Minimum Detection Limit: 31.25 pg/mL

Application: ELISA

Sample Type: Plasma, Serum

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Collagen Type II Alpha 1 (COL2a1). No significant cross-reactivity or interference between Collagen Type II Alpha 1 (COL2a1) and analogues was observed.

Sensitivity: 13.9 pg/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Collagen Type II Alpha 1 (COL2a1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Collagen Type II Alpha 1 (COL2a1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Collagen Type II Alpha 1 (COL2a1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Collagen Type II Alpha 1 (COL2a1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Collagen Type II Alpha 1 (COL2a1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Collagen Type II Alpha 1 (COL2a1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for COL2A1

Target: COL2A1 (Collagen, Type II, alpha 1 (COL2A1))

Alternative Name: Collagen Type II Alpha 1 (COL2A1 Products)

Synonyms: ANFH ELISA Kit, AOM ELISA Kit, COL11A3 ELISA Kit, SEDC ELISA Kit, Col2 ELISA Kit, Col2a ELISA Kit, Col2a-1 ELISA Kit, Del1 ELISA Kit, Dmm ELISA Kit, Lpk ELISA Kit, M100856 ELISA Kit, Rgsc856 ELISA Kit, CG2A1A ELISA Kit, COLLII ELISA Kit, col2a1a ELISA Kit, CB11 ELISA Kit, col2a1 ELISA Kit, coll2a1 ELISA Kit, fb38c06 ELISA Kit, fc10c01 ELISA Kit, wu:fb38c06 ELISA Kit, wu:fc10c01 ELISA Kit, collagen type II alpha 1 chain ELISA Kit, collagen, type II, alpha 1 ELISA Kit, collagen, type II, alpha 1 L homeolog ELISA Kit, collagen, type II, alpha 1 S homeolog ELISA Kit, collagen, type II, alpha 1a ELISA Kit, COL2A1 ELISA Kit, Col2a1 ELISA Kit, col2a1 ELISA Kit, col2a1.L ELISA Kit, col2a1.S ELISA Kit, col2a1a ELISA Kit

Background:

Gene Name: Collagen Type II Alpha 1

Gene Aliases: COL2-A1, COL2A1, SEDC, Chondrocalcin, Collagen Alpha-1(II)Chain, Primary Osteoarthritis,Spondyloepiphyseal Dysplasia,Congenital

Gene ID: 1280

UniProt: P02458

Pathways: Sensory Perception of Sound, Growth Factor Binding