COL11A1 ELISA Kit

Catalog No. ABIN5653149

Product Details COL11A1 ELISA Kit

Target: COL11A1 (Collagen, Type XI, alpha 1 (COL11A1))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 39 pg/mL - 2500 pg/mL

Minimum Detection Limit: 39 pg/mL

Application: ELISA

Sample Type: Plasma, Serum

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Collagen Type XI Alpha 1 (COL11a1). No significant cross-reactivity or interference between Collagen Type XI Alpha 1 (COL11a1) and analogues was observed.

Sensitivity: 15 pg/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Collagen Type XI Alpha 1 (COL11a1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Collagen Type XI Alpha 1 (COL11a1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Collagen Type XI Alpha 1 (COL11a1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Collagen Type XI Alpha 1 (COL11a1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Collagen Type XI Alpha 1 (COL11a1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Collagen Type XI Alpha 1 (COL11a1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for COL11A1

Target: COL11A1 (Collagen, Type XI, alpha 1 (COL11A1))

Alternative Name: Collagen Type XI Alpha 1 (COL11A1 Products)

Synonyms: wu:fb34e11 ELISA Kit, wu:fc11b03 ELISA Kit, zgc:158335 ELISA Kit, col11a1 ELISA Kit, Col11a1 ELISA Kit, CO11A1 ELISA Kit, COLL6 ELISA Kit, STL2 ELISA Kit, C530001D20Rik ELISA Kit, cho ELISA Kit, collagen type XI alpha 1 chain ELISA Kit, collagen, type XI, alpha 1a ELISA Kit, collagen, type V, alpha 3 ELISA Kit, collagen, type XI, alpha 1 ELISA Kit, COL11A1 ELISA Kit, col11a1a ELISA Kit, col5a3 ELISA Kit, Col11a1 ELISA Kit

Background:

Gene Name: Collagen Type XI Alpha 1

Gene Aliases: COL11-A1, CO11A1, COLL6, STL2, Collagen Alpha-1(XI)chain

Gene ID: 1301

UniProt: P12107

Pathways: Sensory Perception of Sound