CTGF CLIA Kit

Catalog No. ABIN5653298

Product Details CTGF CLIA Kit

Target: CTGF (Connective Tissue Growth Factor (CTGF))

Reactivity: Rat

Detection Method: Chemiluminescent

Method Type: Sandwich ELISA

Detection Range: 6.25 pg/mL - 400 pg/mL

Minimum Detection Limit: 6.25 pg/mL

Application: ELISA

Sample Type: Plasma, Serum

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Connective Tissue Growth Factor (CTGF). No significant cross-reactivity or interference between Connective Tissue Growth Factor (CTGF) and analogues was observed.

Sensitivity: 0.22 pg/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 2 - 3 h

Plate: Pre-coated

Protocol: The microplate provided in this kit has been pre-coated with an antibody specific to Connective Tissue Growth Factor (CTGF). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Connective Tissue Growth Factor (CTGF). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Connective Tissue Growth Factor (CTGF) level in the sample or standard.,

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Connective Tissue Growth Factor (CTGF) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Connective Tissue Growth Factor (CTGF) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details

Target: CTGF (Connective Tissue Growth Factor (CTGF))

Alternative Name: Connective Tissue Growth Factor (CTGF Products)

Synonyms: Ccn2 CLIA Kit, Fisp12 CLIA Kit, Hcs24 CLIA Kit, fisp-12 CLIA Kit, CCN2 CLIA Kit, HCS24 CLIA Kit, IGFBP8 CLIA Kit, NOV2 CLIA Kit, CTGRP CLIA Kit, ctgf CLIA Kit, hm:zeh1559 CLIA Kit, si:dkey-266k12.4 CLIA Kit, wu:fl25c03 CLIA Kit, zeh1559 CLIA Kit, zgc:136644 CLIA Kit, XCTGF CLIA Kit, ccn2 CLIA Kit, nov2 CLIA Kit, hcs24 CLIA Kit, igfbp8 CLIA Kit, CTGF CLIA Kit, si:ch211-173b8.2 CLIA Kit, connective tissue growth factor CLIA Kit, connective tissue growth factor a CLIA Kit, connective tissue growth factor L homeolog CLIA Kit, connective tissue growth factor b CLIA Kit, Ctgf CLIA Kit, CTGF CLIA Kit, ctgfa CLIA Kit, ctgf.L CLIA Kit, ctgf CLIA Kit, ctgfb CLIA Kit

Background:

Gene Name: Connective Tissue Growth Factor

Gene Aliases: CCN2, HCS24, IGFBP8, NOV2, Insulin-Like Growth Factor-Binding Protein 8, Hypertrophic Chondrocyte-Specific Protein 24, CCN Family Member 2

Gene ID: 64032

UniProt: Q9R1E9

Pathways: Regulation of Lipid Metabolism by PPARalpha, Positive Regulation of Endopeptidase Activity, Growth Factor Binding