CYP26A1 ELISA Kit

Catalog No. ABIN5653500

Product Details CYP26A1 ELISA Kit

Target: CYP26A1 (Cytochrome P450, Family 26, Subfamily A, Polypeptide 1 (CYP26A1))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.156 ng/mL - 10 ng/mL

Minimum Detection Limit: 0.156 ng/mL

Application: ELISA

Sample Type: Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Cytochrome P450 26A1 (CYP26A1). No significant cross-reactivity or interference between Cytochrome P450 26A1 (CYP26A1) and analogues was observed.

Sensitivity: 0.055 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cytochrome P450 26A1 (CYP26A1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cytochrome P450 26A1 (CYP26A1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cytochrome P450 26A1 (CYP26A1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cytochrome P450 26A1 (CYP26A1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cytochrome P450 26A1 (CYP26A1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cytochrome P450 26A1 (CYP26A1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for CYP26A1

Target: CYP26A1 (Cytochrome P450, Family 26, Subfamily A, Polypeptide 1 (CYP26A1))

Alternative Name: Cytochrome P450 26A1 (CYP26A1 Products)

Synonyms: CP26 ELISA Kit, CYP26 ELISA Kit, P450RAI ELISA Kit, P450RAI1 ELISA Kit, Cyp26 ELISA Kit, P450RA ELISA Kit, RAH ELISA Kit, RACE ELISA Kit, XCYP26A1 ELISA Kit, cyp26 ELISA Kit, xCYP26 ELISA Kit, cp26 ELISA Kit, p450rai ELISA Kit, p450rai1 ELISA Kit, CYP26A ELISA Kit, cb24 ELISA Kit, id:ibd5061 ELISA Kit, wu:fb81e05 ELISA Kit, cytochrome P450 family 26 subfamily A member 1 ELISA Kit, cytochrome P450, family 26, subfamily a, polypeptide 1 ELISA Kit, cytochrome P450 family 26 subfamily A member 1 S homeolog ELISA Kit, cytochrome P450 family 26 subfamily A member 1 L homeolog ELISA Kit, cytochrome P450, family 26, subfamily A, polypeptide 1 ELISA Kit, CYP26A1 ELISA Kit, Cyp26a1 ELISA Kit, cyp26a1.S ELISA Kit, cyp26a1.L ELISA Kit, cyp26a1 ELISA Kit

Background:

Gene Name: Cytochrome P450 26A1

Gene Aliases: CP26, CYP26, P450RAI, P450RAI1, hP450RAI, Cytochrome P450 retinoic acid-inactivating 1, Retinoic acid 4-hydroxylase, Retinoic acid-metabolizing cytochrome

Gene ID: 1592

UniProt: O43174

Pathways: Retinoic Acid Receptor Signaling Pathway, Monocarboxylic Acid Catabolic Process