CYP3A4 ELISA Kit

Catalog No. ABIN5653515

Product Details CYP3A4 ELISA Kit

Target: CYP3A4 (Cytochrome P450, Family 3, Subfamily A, Polypeptide 4 (CYP3A4))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 1.56 ng/mL - 100 ng/mL

Minimum Detection Limit: 1.56 ng/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Cytochrome P450 3A4 (CYP3A4). No significant cross-reactivity or interference between Cytochrome P450 3A4 (CYP3A4) and analogues was observed.

Sensitivity: 0.62 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cytochrome P450 3A4 (CYP3A4). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cytochrome P450 3A4 (CYP3A4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cytochrome P450 3A4 (CYP3A4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cytochrome P450 3A4 (CYP3A4) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cytochrome P450 3A4 (CYP3A4) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cytochrome P450 3A4 (CYP3A4) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for CYP3A4

Target: CYP3A4 (Cytochrome P450, Family 3, Subfamily A, Polypeptide 4 (CYP3A4))

Alternative Name: Cytochrome P450 3A4 (CYP3A4 Products)

Synonyms: CP33 ELISA Kit, CP34 ELISA Kit, CYP3A ELISA Kit, CYP3A3 ELISA Kit, CYPIIIA3 ELISA Kit, CYPIIIA4 ELISA Kit, HLP ELISA Kit, NF-25 ELISA Kit, P450C3 ELISA Kit, P450PCN1 ELISA Kit, MGC108372 ELISA Kit, CYP3A80 ELISA Kit, CYP3A4 ELISA Kit, CYP3A21 ELISA Kit, CYPIIIA21 ELISA Kit, CYP3A12 ELISA Kit, cytochrome P450 family 3 subfamily A member 4 ELISA Kit, cytochrome P450 family 3 subfamily A member 43 ELISA Kit, cytochrome P450 3A4 ELISA Kit, cytochrome P450, subfamily IIIA, polypeptide 4 ELISA Kit, cytochrome P450, subfamily IIIA (niphedipine oxidase), polypeptide 4 ELISA Kit, cytochrome P450, family 3, subfamily A, polypeptide 4 ELISA Kit, CYP3A4 ELISA Kit, CYP3A43 ELISA Kit, PTRG_01782 ELISA Kit, PTRG_06060 ELISA Kit, cyp3a4 ELISA Kit

Background:

Gene Name: Cytochrome P450 3A4

Gene Aliases: CYP3A3, CYPIIIA4, 1,8-cineole 2-exo-monooxygenase, Albendazole sulfoxidase, Nifedipine oxidase, Quinine 3-monooxygenase, Taurochenodeoxycholate 6-alpha-hydroxylase

Pathways: Steroid Hormone Biosynthesis