Defensin, beta 103B (DEFB103B) ELISA Kit

Details for Product No. ABIN5653583, Supplier: Log in to see
Antigen
  • BD-3
  • DEFB103
  • DEFB3
  • HBD3
  • HBP3
  • defensin beta 103B
  • DEFB103B
Reactivity
Human
Alternatives
Kits with alternative reactivity to:
5
Method Type
Sandwich ELISA
Detection Range
31.25 pg/mL - 2000 pg/mL
Minimum Detection Limit
31.25 pg/mL
Application
ELISA
Options
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Sample Type Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Analytical Method Quantitative
Detection Method Colorimetric
Specificity This assay has high sensitivity and excellent specificity for detection of Defensin Beta 103B (DEFB103B). No significant cross-reactivity or interference between Defensin Beta 103B (DEFB103B) and analogues was observed.
Sensitivity 12.7 pg/mL
Antigen
Alternative Name Defensin Beta 103B (DEFB103B ELISA Kit Abstract)
Background

Gene Name: Defensin Beta 103B

Gene Aliases: HBP3, HBD3, BD3, DEFB103, DEFB3, Defensin-like protein, Beta-defensin 3

Pathways Production of Molecular Mediator of Immune Response
Comment

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time 3 h
Plate Pre-coated
Protocol The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Defensin Beta 103B (DEFB103B). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Defensin Beta 103B (DEFB103B). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Defensin Beta 103B (DEFB103B), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Defensin Beta 103B (DEFB103B) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Assay Precision Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Defensin Beta 103B (DEFB103B) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Defensin Beta 103B (DEFB103B) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Restrictions For Research Use only
Handling Advice The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
Storage 4 °C,-20 °C
Storage Comment -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
Expiry Date 4-8 months
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