DHODH ELISA Kit

Catalog No. ABIN5653671

Product Details DHODH ELISA Kit

Target: DHODH (Dihydroorotate Dehydrogenase (DHODH))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.625 ng/mL - 10 ng/mL

Minimum Detection Limit: 0.625 ng/mL

Application: ELISA

Sample Type: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Dihydroorotate Dehydrogenase (DHODH). No significant cross-reactivity or interference between Dihydroorotate Dehydrogenase (DHODH) and analogues was observed.

Sensitivity: 0.253 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dihydroorotate Dehydrogenase (DHODH). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Dihydroorotate Dehydrogenase (DHODH). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Dihydroorotate Dehydrogenase (DHODH), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Dihydroorotate Dehydrogenase (DHODH) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Dihydroorotate Dehydrogenase (DHODH) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Dihydroorotate Dehydrogenase (DHODH) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for DHODH

Target: DHODH (Dihydroorotate Dehydrogenase (DHODH))

Alternative Name: Dihydroorotate Dehydrogenase (DHODH Products)

Synonyms: DHOdehase ELISA Kit, POADS ELISA Kit, URA1 ELISA Kit, 2810417D19Rik ELISA Kit, AI834883 ELISA Kit, DDBDRAFT_0167009 ELISA Kit, DDBDRAFT_0185217 ELISA Kit, DDB_0167009 ELISA Kit, DDB_0185217 ELISA Kit, DHOD ELISA Kit, DHODase ELISA Kit, XFHB_12025 ELISA Kit, dhodh ELISA Kit, dihydroorotate dehydrogenase (quinone) ELISA Kit, dihydroorotate dehydrogenase ELISA Kit, dihydroorotate dehydrogenase (quinone) L homeolog ELISA Kit, DHODH ELISA Kit, Dhodh ELISA Kit, pyrD ELISA Kit, pyr4 ELISA Kit, Mrub_0263 ELISA Kit, Arnit_2124 ELISA Kit, Trad_2703 ELISA Kit, Ftrac_1484 ELISA Kit, Ndas_0799 ELISA Kit, Mesil_1876 ELISA Kit, Slip_0841 ELISA Kit, Spirs_1436 ELISA Kit, XFHB_RS12780 ELISA Kit, dhodh.L ELISA Kit

Background:

Gene Name: Dihydroorotate Dehydrogenase

Gene Aliases: DHOdehase, Dihydroorotate oxidase, Dihydroorotate dehydrogenase (quinone), mitochondrial

Gene ID: 1723

UniProt: Q02127

Pathways: Ribonucleoside Biosynthetic Process, Protein targeting to Nucleus