F4/80 ELISA Kit

Catalog No. ABIN5653785

Product Details F4/80 ELISA Kit

Target: F4/80 (EMR1) (Egf-Like Module Containing, Mucin-Like, Hormone Receptor-Like 1 (EMR1))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 62.5 pg/mL - 4000 pg/mL

Minimum Detection Limit: 62.5 pg/mL

Application: ELISA

Sample Type: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of EGF Like Module Containing Mucin Like Hormone Receptor 1 (EMR1). No significant cross-reactivity or interference between EGF Like Module Containing Mucin Like Hormone Receptor 1 (EMR1) and analogues was observed.

Sensitivity: 25.4 pg/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to EGF Like Module Containing Mucin Like Hormone Receptor 1 (EMR1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to EGF Like Module Containing Mucin Like Hormone Receptor 1 (EMR1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain EGF Like Module Containing Mucin Like Hormone Receptor 1 (EMR1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of EGF Like Module Containing Mucin Like Hormone Receptor 1 (EMR1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level EGF Like Module Containing Mucin Like Hormone Receptor 1 (EMR1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level EGF Like Module Containing Mucin Like Hormone Receptor 1 (EMR1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for F4/80

Target: F4/80 (EMR1) (Egf-Like Module Containing, Mucin-Like, Hormone Receptor-Like 1 (EMR1))

Alternative Name: EGF Like Module Containing Mucin Like Hormone Receptor 1 (EMR1 Products)

Synonyms: EMR1 ELISA Kit, TM7LN3 ELISA Kit, DD7A5-7 ELISA Kit, EGF-TM7 ELISA Kit, F4/80 ELISA Kit, Gpf480 ELISA Kit, Ly71 ELISA Kit, adhesion G protein-coupled receptor E1 ELISA Kit, ADGRE1 ELISA Kit, LOC100424335 ELISA Kit, Adgre1 ELISA Kit

Background:

Gene Name: EGF Like Module Containing Mucin Like Hormone Receptor 1

Gene Aliases: TM7LN3, EGF-like module receptor 1, EMR1 hormone receptor

Gene ID: 2015

UniProt: Q14246