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EPHX4 ELISA Kit

EPHX4 Reactivity: Mouse Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Plasma, Serum
Catalog No. ABIN5653961
  • Target See all EPHX4 products
    EPHX4 (Epoxide Hydrolase 4 (EPHX4))
    Reactivity
    • 2
    • 2
    Mouse
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Detection Range
    62.5 pg/mL - 4000 pg/mL
    Minimum Detection Limit
    62.5 pg/mL
    Application
    ELISA
    Sample Type
    Plasma, Serum
    Analytical Method
    Quantitative
    Specificity
    This assay has high sensitivity and excellent specificity for detection of Epoxide Hydrolase 4 (EPHX4). No significant cross-reactivity or interference between Epoxide Hydrolase 4 (EPHX4) and analogues was observed.
    Sensitivity
    27.2 pg/mL
    Featured
    Discover our best selling EPHX4 ELISA Kit
  • Comment

    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

    Assay Time
    3 h
    Plate
    Pre-coated
    Protocol
    The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Epoxide Hydrolase 4 (EPHX4). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Epoxide Hydrolase 4 (EPHX4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Epoxide Hydrolase 4 (EPHX4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Epoxide Hydrolase 4 (EPHX4) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
    Assay Precision
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Epoxide Hydrolase 4 (EPHX4) were tested 20 times on one plate, respectively
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Epoxide Hydrolase 4 (EPHX4) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%
    Restrictions
    For Research Use only
  • Handling Advice
    The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
    Storage
    4 °C,-20 °C
    Storage Comment
    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
    Expiry Date
    4-8 months
  • Target See all EPHX4 products
    EPHX4 (Epoxide Hydrolase 4 (EPHX4))
    Alternative Name
    Epoxide Hydrolase 4 (EPHX4 Products)
    Synonyms
    ABHD7 ELISA Kit, EPHXRP ELISA Kit, Abhd7 ELISA Kit, Ephxrp ELISA Kit, Gm1382 ELISA Kit, epoxide hydrolase 4 ELISA Kit, EPHX4 ELISA Kit, Ephx4 ELISA Kit
    Background

    Gene Name: Epoxide Hydrolase 4

    Gene Aliases: ABHD7, EPHXRP, Abhydrolase Domain Containing 7, Epoxide hydrolase-related protein

    Gene ID
    384214
    UniProt
    Q6IE26
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