ESR2 CLIA Kit

Catalog No. ABIN5653990

Product Details ESR2 CLIA Kit

Target: ESR2 (Estrogen Receptor 2 (ESR2))

Reactivity: Human

Detection Method: Chemiluminescent

Method Type: Sandwich ELISA

Detection Range: 312.5 pg/mL - 20000 pg/mL

Minimum Detection Limit: 312.5 pg/mL

Application: ELISA

Sample Type: Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Estrogen Receptor Beta (ERb). No significant cross-reactivity or interference between Estrogen Receptor Beta (ERb) and analogues was observed.

Sensitivity: 10.17 pg/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 2 - 3 h

Plate: Pre-coated

Protocol: The microplate provided in this kit has been pre-coated with an antibody specific to Estrogen Receptor Beta (ERb). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Estrogen Receptor Beta (ERb). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Estrogen Receptor Beta (ERb) level in the sample or standard.,

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Estrogen Receptor Beta (ERb) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Estrogen Receptor Beta (ERb) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details

Target: ESR2 (Estrogen Receptor 2 (ESR2))

Alternative Name: Estrogen Receptor Beta (ESR2 Products)

Synonyms: ER-BETA CLIA Kit, ESR-BETA CLIA Kit, ESRB CLIA Kit, ESTRB CLIA Kit, Erb CLIA Kit, NR3A2 CLIA Kit, ER[b]1 CLIA Kit, zfER-beta1 CLIA Kit, zfER[b]1 CLIA Kit, erb2 CLIA Kit, CERB CLIA Kit, ERB CLIA Kit, erb CLIA Kit, esrb CLIA Kit, estrb CLIA Kit, nr3a2 CLIA Kit, ERbeta CLIA Kit, er-beta CLIA Kit, esr-beta CLIA Kit, ESR2 CLIA Kit, ERBETA CLIA Kit, er CLIA Kit, ER-beta CLIA Kit, ER[b] CLIA Kit, Estrb CLIA Kit, ER[b]2 CLIA Kit, ER[b]a CLIA Kit, zfER[b]2 CLIA Kit, zfER-beta2 CLIA Kit, Erb2 CLIA Kit, esr2 CLIA Kit, erbeta CLIA Kit, Esr2 CLIA Kit, ERBeta2 CLIA Kit, estrogen receptor 2 CLIA Kit, estrogen receptor 2b CLIA Kit, estrogen receptor beta 2 CLIA Kit, estrogen receptor 2 (beta) CLIA Kit, estrogen receptor 2a CLIA Kit, estrogen receptor beta CLIA Kit, ESR2 CLIA Kit, esr2b CLIA Kit, erb2 CLIA Kit, esr2 CLIA Kit, Esr2 CLIA Kit, esr2a CLIA Kit, erbeta CLIA Kit

Background:

Gene Name: Estrogen Receptor Beta

Gene Aliases: ER-B, ESRB, ESTRB, NR3A2, NR3-A2, ESR2, Estrogen Receptor 2, Nuclear Receptor Subfamily 3,Group A,Member 2

Gene ID: 2100

UniProt: Q92731

Pathways: Nuclear Receptor Transcription Pathway, EGFR Signaling Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling