ESR2 ELISA Kit

Catalog No. ABIN5653993

Product Details ESR2 ELISA Kit

Target: ESR2 (Estrogen Receptor 2 (ESR2))

Reactivity: Rat

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.312 ng/mL - 20 ng/mL

Minimum Detection Limit: 0.312 ng/mL

Application: ELISA

Sample Type: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Estrogen Receptor Beta (ERb). No significant cross-reactivity or interference between Estrogen Receptor Beta (ERb) and analogues was observed.

Sensitivity: 0.119 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Estrogen Receptor Beta (ERb). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Estrogen Receptor Beta (ERb). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Estrogen Receptor Beta (ERb), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Estrogen Receptor Beta (ERb) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Estrogen Receptor Beta (ERb) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Estrogen Receptor Beta (ERb) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for ESR2

Target: ESR2 (Estrogen Receptor 2 (ESR2))

Alternative Name: Estrogen Receptor Beta (ESR2 Products)

Synonyms: ER-BETA ELISA Kit, ESR-BETA ELISA Kit, ESRB ELISA Kit, ESTRB ELISA Kit, Erb ELISA Kit, NR3A2 ELISA Kit, ER[b]1 ELISA Kit, zfER-beta1 ELISA Kit, zfER[b]1 ELISA Kit, erb2 ELISA Kit, CERB ELISA Kit, ERB ELISA Kit, erb ELISA Kit, esrb ELISA Kit, estrb ELISA Kit, nr3a2 ELISA Kit, ERbeta ELISA Kit, er-beta ELISA Kit, esr-beta ELISA Kit, ESR2 ELISA Kit, ERBETA ELISA Kit, er ELISA Kit, ER-beta ELISA Kit, ER[b] ELISA Kit, Estrb ELISA Kit, ER[b]2 ELISA Kit, ER[b]a ELISA Kit, zfER[b]2 ELISA Kit, zfER-beta2 ELISA Kit, Erb2 ELISA Kit, esr2 ELISA Kit, erbeta ELISA Kit, Esr2 ELISA Kit, ERBeta2 ELISA Kit, estrogen receptor 2 ELISA Kit, estrogen receptor 2b ELISA Kit, estrogen receptor beta 2 ELISA Kit, estrogen receptor 2 (beta) ELISA Kit, estrogen receptor 2a ELISA Kit, estrogen receptor beta ELISA Kit, ESR2 ELISA Kit, esr2b ELISA Kit, erb2 ELISA Kit, esr2 ELISA Kit, Esr2 ELISA Kit, esr2a ELISA Kit, erbeta ELISA Kit

Background:

Gene Name: Estrogen Receptor Beta

Gene Aliases: ER-B, ESRB, ESTRB, NR3A2, NR3-A2, ESR2, Estrogen Receptor 2, Nuclear Receptor Subfamily 3,Group A,Member 2

Pathways: Nuclear Receptor Transcription Pathway, EGFR Signaling Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling