Fatty Acid Synthase CLIA Kit

Catalog No. ABIN5654091

Product Details Fatty Acid Synthase CLIA Kit

Target: Fatty Acid Synthase (FASN)

Reactivity: Chicken

Detection Method: Chemiluminescent

Method Type: Sandwich ELISA

Detection Range: 62.5 pg/mL - 4000 pg/mL

Minimum Detection Limit: 62.5 pg/mL

Application: ELISA

Sample Type: Plasma, Serum

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Fatty Acid Synthase (FASN). No significant cross-reactivity or interference between Fatty Acid Synthase (FASN) and analogues was observed.

Sensitivity: 2.37 pg/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 2 - 3 h

Plate: Pre-coated

Protocol: The microplate provided in this kit has been pre-coated with an antibody specific to Fatty Acid Synthase (FASN). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Fatty Acid Synthase (FASN). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Fatty Acid Synthase (FASN) level in the sample or standard.,

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Fatty Acid Synthase (FASN) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Fatty Acid Synthase (FASN) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details

Target: Fatty Acid Synthase (FASN)

Alternative Name: Fatty Acid Synthase (FASN Products)

Synonyms: FAS CLIA Kit, OA-519 CLIA Kit, SDR27X1 CLIA Kit, FASN CLIA Kit, fj34h12 CLIA Kit, wu:fj34h12 CLIA Kit, Cgl0836 CLIA Kit, A630082H08Rik CLIA Kit, BcDNA:GH07626 CLIA Kit, BcDNA:gh07626 CLIA Kit, DM_7295848 CLIA Kit, Dmel\\CG3523 CLIA Kit, Fatty acid synthase CLIA Kit, anon-EST:Posey235 CLIA Kit, anon-EST:Posey43 CLIA Kit, anon-WO0138581.1 CLIA Kit, dFAS CLIA Kit, fas CLIA Kit, fatty acid synthase CLIA Kit, fatty acid synthase Fas CLIA Kit, type I polyketide synthase CLIA Kit, Fatty acid synthase Fas CLIA Kit, Fatty Acid SyNthase CLIA Kit, fatty-acid synthase CLIA Kit, fatty-acid synthase II CLIA Kit, fatty-acid synthase fas CLIA Kit, Fatty acid synthase 1 CLIA Kit, FASN CLIA Kit, fas CLIA Kit, Fasn CLIA Kit, BAD_RS01360 CLIA Kit, fasn CLIA Kit, fasn-1 CLIA Kit, NCgl0802 CLIA Kit, fasB CLIA Kit, MT2600 CLIA Kit, TBMG_01448 CLIA Kit, FASN1 CLIA Kit

Background:

Gene Name: Fatty Acid Synthase

Gene Aliases: FAS, OA519, SDR27X1, Short Chain Dehydrogenase/Reductase Family 27X,Member 1, S-acetyltransferase, 3-oxoacyl synthase, Oleoyl hydrolase, Enoyl-acyl-carrier-protein reductase

UniProt: P12276

Pathways: AMPK Signaling