SLC27A5 ELISA Kit

Catalog No. ABIN5654098

Product Details SLC27A5 ELISA Kit

Target: SLC27A5 (Solute Carrier Family 27 (Fatty Acid Transporter), Member 5 (SLC27A5))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.156 ng/mL - 10 ng/mL

Minimum Detection Limit: 0.156 ng/mL

Application: ELISA

Sample Type: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Fatty Acid Transport Protein 5 (FATP5). No significant cross-reactivity or interference between Fatty Acid Transport Protein 5 (FATP5) and analogues was observed.

Sensitivity: 0.054 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Fatty Acid Transport Protein 5 (FATP5). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Fatty Acid Transport Protein 5 (FATP5). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Fatty Acid Transport Protein 5 (FATP5), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Fatty Acid Transport Protein 5 (FATP5) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Fatty Acid Transport Protein 5 (FATP5) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Fatty Acid Transport Protein 5 (FATP5) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for SLC27A5

Target: SLC27A5 (Solute Carrier Family 27 (Fatty Acid Transporter), Member 5 (SLC27A5))

Alternative Name: Fatty Acid Transport Protein 5 (SLC27A5 Products)

Synonyms: ACSB ELISA Kit, ACSVL6 ELISA Kit, BACS ELISA Kit, BAL ELISA Kit, FACVL3 ELISA Kit, FATP-5 ELISA Kit, FATP5 ELISA Kit, VLACSR ELISA Kit, VLCS-H2 ELISA Kit, VLCSH2 ELISA Kit, Vlacsr ELISA Kit, rBAL-1 ELISA Kit, SLC27A5 ELISA Kit, solute carrier family 27 member 5 ELISA Kit, solute carrier family 27 (fatty acid transporter), member 5 ELISA Kit, bile acyl-CoA synthetase ELISA Kit, SLC27A5 ELISA Kit, Slc27a5 ELISA Kit, LOC608675 ELISA Kit, LOC100450572 ELISA Kit

Background:

Gene Name: Fatty Acid Transport Protein 5

Gene Aliases: SLC27A5, ACSB, ACSVL6, FACVL3, VLACSR, BACS, VLCSH2, Fatty Acid Coenzyme A Ligase Very Long Chain 3, Solute Carrier Family 27 Member 5, Bile Acyl CoA Synthetase

Gene ID: 26459

UniProt: Q4LDG0