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FGF1 CLIA Kit

FGF1 Reactivity: Cow Chemiluminescent Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Plasma, Serum
Catalog No. ABIN5654173
  • Target See all FGF1 CLIA Kits
    FGF1 (Fibroblast Growth Factor 1 (Acidic) (FGF1))
    Reactivity
    • 6
    • 5
    • 4
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    Cow
    Detection Method
    Chemiluminescent
    Method Type
    Sandwich ELISA
    Detection Range
    62.5 pg/mL - 4000 pg/mL
    Minimum Detection Limit
    62.5 pg/mL
    Application
    ELISA
    Sample Type
    Plasma, Serum
    Analytical Method
    Quantitative
    Specificity
    This assay has high sensitivity and excellent specificity for detection of Fibroblast Growth Factor 1, Acidic (FGF1). No significant cross-reactivity or interference between Fibroblast Growth Factor 1, Acidic (FGF1) and analogues was observed.
    Sensitivity
    2.45 pg/mL
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  • Comment

    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

    Assay Time
    2 - 3 h
    Plate
    Pre-coated
    Protocol
    The microplate provided in this kit has been pre-coated with an antibody specific to Fibroblast Growth Factor 1, Acidic (FGF1). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Fibroblast Growth Factor 1, Acidic (FGF1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Fibroblast Growth Factor 1, Acidic (FGF1) level in the sample or standard.,
    Assay Precision
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Fibroblast Growth Factor 1, Acidic (FGF1) were tested 20 times on one plate, respectively
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Fibroblast Growth Factor 1, Acidic (FGF1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%
    Restrictions
    For Research Use only
  • Handling Advice
    Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
    Storage
    4 °C,-20 °C
    Storage Comment
    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
    Expiry Date
    4-8 months
  • Target See all FGF1 CLIA Kits
    FGF1 (Fibroblast Growth Factor 1 (Acidic) (FGF1))
    Alternative Name
    Fibroblast Growth Factor 1, Acidic (FGF1 Products)
    Synonyms
    AFGF CLIA Kit, ECGF CLIA Kit, ECGF-beta CLIA Kit, ECGFA CLIA Kit, ECGFB CLIA Kit, FGF-1 CLIA Kit, FGF-alpha CLIA Kit, FGFA CLIA Kit, GLIO703 CLIA Kit, HBGF-1 CLIA Kit, HBGF1 CLIA Kit, Fgf1 CLIA Kit, FGF1 CLIA Kit, aFGF CLIA Kit, ecgf CLIA Kit, fgfa CLIA Kit, ecgfa CLIA Kit, ecgfb CLIA Kit, fgf-1 CLIA Kit, hbgf1 CLIA Kit, glio703 CLIA Kit, ecgf-beta CLIA Kit, fgf-alpha CLIA Kit, Dffrx CLIA Kit, Fam CLIA Kit, Fgf-1 CLIA Kit, Fgfa CLIA Kit, zgc:136885 CLIA Kit, LOC100221393 CLIA Kit, fgf1 CLIA Kit, zgc:73249 CLIA Kit, fibroblast growth factor 1 CLIA Kit, fibroblast growth factor 1 L homeolog CLIA Kit, fibroblast growth factor 1b CLIA Kit, fibroblast growth factor 1a CLIA Kit, FGF1 CLIA Kit, fgf1.L CLIA Kit, fgf1 CLIA Kit, Fgf1 CLIA Kit, fgf1b CLIA Kit, LOC100221393 CLIA Kit, fgf1a CLIA Kit
    Background

    Gene Name: Fibroblast Growth Factor 1, Acidic

    Gene Aliases: ECGF, AFGF, ECGFA, ECGFB, FGF-Alpha, FGFA, HBGF1, ECGFB, HBGF1, Acidic Fibroblast Growth Factor, heparin-binding growth factor 1, Endothelial Cell Growth Factor, Beta

    Pathways
    RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway
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