FGF21 ELISA Kit (Fibroblast Growth Factor 21)

Details for Product FGF21 ELISA Kit No. ABIN5654242, Supplier: Log in to see
Antigen
  • FGF21
  • fibroblast growth factor 21
  • FGF21
  • fgf21
  • Fgf21
Reactivity
Rat (Rattus)
Alternatives
Kits with alternative reactivity to:
30
24
16
15
5
2
2
2
2
2
1
1
1
Method Type
Competition ELISA
Detection Range
1.17 pg/mL - 300 pg/mL
Minimum Detection Limit
1.17 pg/mL
Application
ELISA
Options
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Sample Type Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Analytical Method Quantitative
Detection Method Chemiluminescent
Specificity This assay has high sensitivity and excellent specificity for detection of Fibroblast Growth Factor 21 (FGF21). No significant cross-reactivity or interference between Fibroblast Growth Factor 21 (FGF21) and analogues was observed.
Sensitivity 0.43 pg/mL
Alternative Name Fibroblast Growth Factor 21 (FGF21 ELISA Kit Abstract)
Background

Gene Name: Fibroblast Growth Factor 21

Gene ID 56636
UniProt Q9JJN1
Pathways RTK Signaling
Comment

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time 2 h
Plate Pre-coated
Protocol The microplate provided in this kit has been pre-coated with a monoclonal antibody specific to Fibroblast Growth Factor 21 (FGF21). A competitive inhibition reaction is launched between biotin labeled Fibroblast Growth Factor 21 (FGF21) and unlabeled Fibroblast Growth Factor 21 (FGF21) (Standards or samples) with the pre-coated antibody specific to Fibroblast Growth Factor 21 (FGF21). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Fibroblast Growth Factor 21 (FGF21) in the sample. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is reverse proportional to the Fibroblast Growth Factor 21 (FGF21) level in the sample or standard.
Assay Precision Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Fibroblast Growth Factor 21 (FGF21) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Fibroblast Growth Factor 21 (FGF21) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Restrictions For Research Use only
Handling Advice Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
Storage 4 °C,-20 °C
Storage Comment -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
Expiry Date 4-8 months
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