LGALS1/Galectin 1 CLIA Kit

Catalog No. ABIN5654476

Product Details LGALS1/Galectin 1 CLIA Kit

Target: LGALS1/Galectin 1 (LGALS1) (Lectin, Galactoside-Binding, Soluble, 1 (LGALS1))

Reactivity: Human

Detection Method: Chemiluminescent

Method Type: Sandwich ELISA

Detection Range: 0.781 ng/mL - 50 ng/mL

Minimum Detection Limit: 0.781 ng/mL

Application: ELISA

Sample Type: Plasma, Serum

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Galectin 1 (GAL1). No significant cross-reactivity or interference between Galectin 1 (GAL1) and analogues was observed.

Sensitivity: 0.03 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 2 - 3 h

Plate: Pre-coated

Protocol: The microplate provided in this kit has been pre-coated with an antibody specific to Galectin 1 (GAL1). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Galectin 1 (GAL1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Galectin 1 (GAL1) level in the sample or standard.,

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Galectin 1 (GAL1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Galectin 1 (GAL1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details

Target: LGALS1/Galectin 1 (LGALS1) (Lectin, Galactoside-Binding, Soluble, 1 (LGALS1))

Alternative Name: Galectin 1 (LGALS1 Products)

Synonyms: GAL1 CLIA Kit, GBP CLIA Kit, Galectin-1 CLIA Kit, LGAS1 CLIA Kit, LGALS1 CLIA Kit, MGC64502 CLIA Kit, GB10026 CLIA Kit, AA410090 CLIA Kit, Gal-1 CLIA Kit, Galbp CLIA Kit, L-14.5 CLIA Kit, L14 CLIA Kit, Lect14 CLIA Kit, galectin-1 CLIA Kit, Galaptin CLIA Kit, galectin 1 CLIA Kit, galectin-1 CLIA Kit, lectin, galactoside-binding, soluble, 1 L homeolog CLIA Kit, protein enabled CLIA Kit, lectin, galactose binding, soluble 1 CLIA Kit, LGALS1 CLIA Kit, Lgals1 CLIA Kit, CC1G_05003 CLIA Kit, CC1G_05004 CLIA Kit, lgals1.L CLIA Kit, LOC408848 CLIA Kit

Background:

Gene Name: Galectin 1

Gene Aliases: LGALS1, GBP, HLBP14, Galaptin, Lectin,Galactoside-Binding Soluble 1, 14 kDa laminin-binding protein, Lactose-binding lectin 1, Putative MAPK-activating protein PM12, S-Lac lectin

UniProt: P09382

Pathways: Carbohydrate Homeostasis