GRB10 CLIA Kit

Catalog No. ABIN5654875

Product Details GRB10 CLIA Kit

Target: GRB10 (Growth Factor Receptor-Bound Protein 10 (GRB10))

Reactivity: Human

Detection Method: Chemiluminescent

Method Type: Sandwich ELISA

Detection Range: 312.5 pg/mL - 20000 pg/mL

Minimum Detection Limit: 312.5 pg/mL

Application: ELISA

Sample Type: Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Growth Factor Receptor Bound Protein 10 (Grb10). No significant cross-reactivity or interference between Growth Factor Receptor Bound Protein 10 (Grb10) and analogues was observed.

Sensitivity: 10.02 pg/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 2 - 3 h

Plate: Pre-coated

Protocol: The microplate provided in this kit has been pre-coated with an antibody specific to Growth Factor Receptor Bound Protein 10 (Grb10). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Growth Factor Receptor Bound Protein 10 (Grb10). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Growth Factor Receptor Bound Protein 10 (Grb10) level in the sample or standard.,

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Growth Factor Receptor Bound Protein 10 (Grb10) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Growth Factor Receptor Bound Protein 10 (Grb10) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details

Target: GRB10 (Growth Factor Receptor-Bound Protein 10 (GRB10))

Alternative Name: Growth Factor Receptor Bound Protein 10 (GRB10 Products)

Synonyms: grb10 CLIA Kit, zgc:91987 CLIA Kit, GRB10 CLIA Kit, grb-10 CLIA Kit, grb-ir CLIA Kit, irbp CLIA Kit, meg1 CLIA Kit, rss CLIA Kit, LOC100224775 CLIA Kit, 5730571D09Rik CLIA Kit, AI325020 CLIA Kit, Meg1 CLIA Kit, mKIAA0207 CLIA Kit, RGD1566234 CLIA Kit, GRB-IR CLIA Kit, Grb-10 CLIA Kit, IRBP CLIA Kit, MEG1 CLIA Kit, RSS CLIA Kit, growth factor receptor bound protein 10 CLIA Kit, growth factor receptor-bound protein 10a CLIA Kit, growth factor receptor bound protein 10 L homeolog CLIA Kit, growth factor receptor-bound protein 10 CLIA Kit, GRB10 CLIA Kit, grb10a CLIA Kit, grb10 CLIA Kit, grb10.L CLIA Kit, LOC100434903 CLIA Kit, Grb10 CLIA Kit

Background:

Gene Name: Growth Factor Receptor Bound Protein 10

Gene Aliases: GRB-IR, IRBP, MEG1, RSS, GRB10 adapter protein, Insulin receptor-binding protein Grb-IR

Gene ID: 2887

UniProt: Q13322

Pathways: Regulation of Carbohydrate Metabolic Process, Signaling Events mediated by VEGFR1 and VEGFR2