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HNF1B CLIA Kit

HNF1B Reactivity: Rat Chemiluminescent Sandwich ELISA 156.25 pg/mL - 10000 pg/mL Tissue Homogenate
Catalog No. ABIN5655091
  • Target See all HNF1B CLIA Kits
    HNF1B (HNF1 Homeobox B (HNF1B))
    Reactivity
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Rat
    Detection Method
    Chemiluminescent
    Method Type
    Sandwich ELISA
    Detection Range
    156.25 pg/mL - 10000 pg/mL
    Minimum Detection Limit
    156.25 pg/mL
    Application
    ELISA
    Sample Type
    Tissue Homogenate
    Analytical Method
    Quantitative
    Specificity
    This assay has high sensitivity and excellent specificity for detection of Hepatocyte Nuclear Factor 1 Beta (HNF1b). No significant cross-reactivity or interference between Hepatocyte Nuclear Factor 1 Beta (HNF1b) and analogues was observed.
    Sensitivity
    6.36 pg/mL
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  • Comment

    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

    Assay Time
    2 - 3 h
    Plate
    Pre-coated
    Protocol
    The microplate provided in this kit has been pre-coated with an antibody specific to Hepatocyte Nuclear Factor 1 Beta (HNF1b). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Hepatocyte Nuclear Factor 1 Beta (HNF1b). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Hepatocyte Nuclear Factor 1 Beta (HNF1b) level in the sample or standard.,
    Assay Precision
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Hepatocyte Nuclear Factor 1 Beta (HNF1b) were tested 20 times on one plate, respectively
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Hepatocyte Nuclear Factor 1 Beta (HNF1b) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%
    Restrictions
    For Research Use only
  • Handling Advice
    Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
    Storage
    4 °C,-20 °C
    Storage Comment
    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
    Expiry Date
    4-8 months
  • Target See all HNF1B CLIA Kits
    HNF1B (HNF1 Homeobox B (HNF1B))
    Alternative Name
    Hepatocyte Nuclear Factor 1 Beta (HNF1B Products)
    Synonyms
    FJHN CLIA Kit, HNF-1B CLIA Kit, HNF1beta CLIA Kit, HNF2 CLIA Kit, HPC11 CLIA Kit, LF-B3 CLIA Kit, LFB3 CLIA Kit, MODY5 CLIA Kit, TCF-2 CLIA Kit, TCF2 CLIA Kit, VHNF1 CLIA Kit, HNF-1b CLIA Kit, HNF1B CLIA Kit, AI385728 CLIA Kit, AI987804 CLIA Kit, HNF-1Beta CLIA Kit, Hnf1beta CLIA Kit, Tcf-2 CLIA Kit, Tcf2 CLIA Kit, vHNF1 CLIA Kit, vHNF-1 CLIA Kit, chunp6877 CLIA Kit, hnf1b CLIA Kit, mst CLIA Kit, tcf2 CLIA Kit, vhnf1 CLIA Kit, lfb3 CLIA Kit, hnf-1beta CLIA Kit, hnf1-beta CLIA Kit, hnf1beta CLIA Kit, HNF1g CLIA Kit, wu:fc23f06 CLIA Kit, HNF1 homeobox B CLIA Kit, HNF1 homeobox Ba CLIA Kit, HNF1 homeobox B S homeolog CLIA Kit, HNF1 homeobox B L homeolog CLIA Kit, HNF1 homeobox Bb CLIA Kit, HNF1B CLIA Kit, Hnf1b CLIA Kit, hnf1ba CLIA Kit, hnf1b.S CLIA Kit, hnf1b.L CLIA Kit, hnf1bb CLIA Kit
    Background

    Gene Name: Hepatocyte Nuclear Factor 1 Beta

    Gene Aliases: FJHN, TCF2, HNF1beta, HNF2, LFB3, LF-B3, MODY5, VHNF1, Transcription Factor 2,Hepatic, Variant Hepatic Nuclear Factor, Homeoprotein LFB3

    Pathways
    Hormone Transport, Stem Cell Maintenance, Tube Formation
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