HMGB1 CLIA Kit

Catalog No. ABIN5655131

Product Details HMGB1 CLIA Kit

Target: HMGB1 (High Mobility Group Box 1 (HMGB1))

Reactivity: Human

Detection Method: Chemiluminescent

Method Type: Sandwich ELISA

Detection Range: 156.25 pg/mL - 10000 pg/mL

Minimum Detection Limit: 156.25 pg/mL

Application: ELISA

Sample Type: Plasma, Serum

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of High Mobility Group Protein 1 (HMG1). No significant cross-reactivity or interference between High Mobility Group Protein 1 (HMG1) and analogues was observed.

Sensitivity: 5.2 pg/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 2 - 3 h

Plate: Pre-coated

Protocol: The microplate provided in this kit has been pre-coated with an antibody specific to High Mobility Group Protein 1 (HMG1). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to High Mobility Group Protein 1 (HMG1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the High Mobility Group Protein 1 (HMG1) level in the sample or standard.,

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level High Mobility Group Protein 1 (HMG1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level High Mobility Group Protein 1 (HMG1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details

Target: HMGB1 (High Mobility Group Box 1 (HMGB1))

Alternative Name: High Mobility Group Protein 1 (HMGB1 Products)

Synonyms: HMG1 CLIA Kit, HMG3 CLIA Kit, SBP-1 CLIA Kit, DEF CLIA Kit, HMG-1 CLIA Kit, Hmg1 CLIA Kit, amphoterin CLIA Kit, p30 CLIA Kit, hmgb1 CLIA Kit, ik:tdsubc_1a5 CLIA Kit, wu:fb23c02 CLIA Kit, xx:tdsubc_1a5 CLIA Kit, zgc:56110 CLIA Kit, zgc:77104 CLIA Kit, hmg-1 CLIA Kit, hmg3 CLIA Kit, sbp-1 CLIA Kit, hmg1 CLIA Kit, HMGB1 CLIA Kit, Ac2-008 CLIA Kit, high mobility group box 1 CLIA Kit, high-mobility group box 1 CLIA Kit, high mobility group box 1a CLIA Kit, high mobility group box 1 L homeolog CLIA Kit, high mobility group protein B1 CLIA Kit, HMGB1 CLIA Kit, Hmgb1 CLIA Kit, hmgb1 CLIA Kit, hmgb1a CLIA Kit, hmgb1.L CLIA Kit, LOC100359149 CLIA Kit

Background:

Gene Name: High Mobility Group Protein 1

Gene Aliases: HMGB1, HMG3, SBP1, Sulfoglucuronyl Carbohydrate Binding Protein, Amphoterin, High Mobility Group Box 1 Protein

Gene ID: 3146

UniProt: P09429

Pathways: p53 Signaling, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Toll-Like Receptors Cascades, Smooth Muscle Cell Migration, Inflammasome