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XIAP CLIA Kit

XIAP Reactivity: Human Chemiluminescent Sandwich ELISA 156.25 pg/mL - 10000 pg/mL Tissue Homogenate
Catalog No. ABIN5655358
  • Target See all XIAP CLIA Kits
    XIAP (X-Linked Inhibitor of Apoptosis (XIAP))
    Reactivity
    • 5
    • 5
    • 3
    Human
    Detection Method
    Chemiluminescent
    Method Type
    Sandwich ELISA
    Detection Range
    156.25 pg/mL - 10000 pg/mL
    Minimum Detection Limit
    156.25 pg/mL
    Application
    ELISA
    Sample Type
    Tissue Homogenate
    Analytical Method
    Quantitative
    Specificity
    This assay has high sensitivity and excellent specificity for detection of Inhibitor Of Apoptosis Protein, X-Linked (XIAP). No significant cross-reactivity or interference between Inhibitor Of Apoptosis Protein, X-Linked (XIAP) and analogues was observed.
    Sensitivity
    4.81 pg/mL
    Top Product
    Discover our top product XIAP ELISA Kit
  • Comment

    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

    Assay Time
    2 - 3 h
    Plate
    Pre-coated
    Protocol
    The microplate provided in this kit has been pre-coated with an antibody specific to Inhibitor Of Apoptosis Protein, X-Linked (XIAP). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Inhibitor Of Apoptosis Protein, X-Linked (XIAP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Inhibitor Of Apoptosis Protein, X-Linked (XIAP) level in the sample or standard.,
    Assay Precision
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Inhibitor Of Apoptosis Protein, X-Linked (XIAP) were tested 20 times on one plate, respectively
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Inhibitor Of Apoptosis Protein, X-Linked (XIAP) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%
    Restrictions
    For Research Use only
  • Handling Advice
    Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
    Storage
    4 °C,-20 °C
    Storage Comment
    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
    Expiry Date
    4-8 months
  • Target See all XIAP CLIA Kits
    XIAP (X-Linked Inhibitor of Apoptosis (XIAP))
    Alternative Name
    Inhibitor Of Apoptosis Protein, X-Linked (XIAP Products)
    Synonyms
    API3 CLIA Kit, BIRC4 CLIA Kit, IAP-3 CLIA Kit, ILP1 CLIA Kit, MIHA CLIA Kit, XLP2 CLIA Kit, hIAP-3 CLIA Kit, hIAP3 CLIA Kit, api3 CLIA Kit, birc4 CLIA Kit, ilp1 CLIA Kit, miha CLIA Kit, xlp2 CLIA Kit, xxiap CLIA Kit, 1110015C02Rik CLIA Kit, Aipa CLIA Kit, Api3 CLIA Kit, Birc4 CLIA Kit, IAP3 CLIA Kit, ILP-1 CLIA Kit, mXIAP CLIA Kit, riap3 CLIA Kit, fb73d01 CLIA Kit, wu:fb73d01 CLIA Kit, zgc:91873 CLIA Kit, BIRC8 CLIA Kit, X-linked inhibitor of apoptosis CLIA Kit, X-linked inhibitor of apoptosis L homeolog CLIA Kit, XIAP CLIA Kit, xiap CLIA Kit, xiap.L CLIA Kit, Xiap CLIA Kit
    Background

    Gene Name: Inhibitor Of Apoptosis Protein, X-Linked

    Gene Aliases: BIRC4, API3, ILP1, MIHA, XLP2, Baculoviral IAP Repeat-Containing 4, E3 ubiquitin-protein ligase XIAP, IAP-like, Inhibitor of apoptosis protein 3, X-linked inhibitor of apoptosis protein

    Gene ID
    331
    UniProt
    P98170
    Pathways
    Apoptosis, Caspase Cascade in Apoptosis, Transition Metal Ion Homeostasis
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