IKBKB ELISA Kit

Catalog No. ABIN5655360

Product Details IKBKB ELISA Kit

Target: IKBKB (Inhibitor of kappa Light Polypeptide Gene Enhancer in B-Cells, Kinase beta (IKBKB))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.156 ng/mL - 10 ng/mL

Minimum Detection Limit: 0.156 ng/mL

Application: ELISA

Sample Type: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Inhibitor Of Kappa-Light Polypeptide Gene Enhancer In B-Cells Kinase Beta (IkBKb). No significant cross-reactivity or interference between Inhibitor Of Kappa-Light Polypeptide Gene Enhancer In B-Cells Kinase Beta (IkBKb) and analogues was observed.

Sensitivity: 0.059 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Inhibitor Of Kappa-Light Polypeptide Gene Enhancer In B-Cells Kinase Beta (IkBKb). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Inhibitor Of Kappa-Light Polypeptide Gene Enhancer In B-Cells Kinase Beta (IkBKb). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Inhibitor Of Kappa-Light Polypeptide Gene Enhancer In B-Cells Kinase Beta (IkBKb), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Inhibitor Of Kappa-Light Polypeptide Gene Enhancer In B-Cells Kinase Beta (IkBKb) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Inhibitor Of Kappa-Light Polypeptide Gene Enhancer In B-Cells Kinase Beta (IkBKb) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Inhibitor Of Kappa-Light Polypeptide Gene Enhancer In B-Cells Kinase Beta (IkBKb) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for IKBKB

Target: IKBKB (Inhibitor of kappa Light Polypeptide Gene Enhancer in B-Cells, Kinase beta (IKBKB))

Alternative Name: Inhibitor Of Kappa-Light Polypeptide Gene Enhancer In B-Cells Kinase Beta (IKBKB Products)

Synonyms: ikk-beta ELISA Kit, ikk2 ELISA Kit, ikkb ELISA Kit, ikkbeta ELISA Kit, IKBKB ELISA Kit, IKKB ELISA Kit, CG4201 ELISA Kit, DIK ELISA Kit, DLAK ELISA Kit, DmIKK ELISA Kit, DmIKKBeta ELISA Kit, DmIKKb ELISA Kit, DmIKKbeta ELISA Kit, DmIkkbeta ELISA Kit, Dmel\\CG4201 ELISA Kit, Dmikkb ELISA Kit, Dmikkbeta ELISA Kit, IK ELISA Kit, IKK ELISA Kit, IKK-beta ELISA Kit, IKKb ELISA Kit, IKKbeta ELISA Kit, IRD-5 ELISA Kit, IRD5 ELISA Kit, Ik ELISA Kit, Ikkb ELISA Kit, Ikkbeta ELISA Kit, Ird-5 ELISA Kit, Ird5 ELISA Kit, LRK ELISA Kit, Lak ELISA Kit, anon-89Bd ELISA Kit, dIKK ELISA Kit, dIKK-beta ELISA Kit, dIKKbeta ELISA Kit, dLak ELISA Kit, dmIKKbeta ELISA Kit, ikk ELISA Kit, ird ELISA Kit, ird5/DmIkk[beta] ELISA Kit, IKK2 ELISA Kit, NFKBIKB ELISA Kit, AIM-1 ELISA Kit, AI132552 ELISA Kit, IKK-2 ELISA Kit, IKK[b] ELISA Kit, inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase beta L homeolog ELISA Kit, inhibitor of nuclear factor kappa B kinase subunit beta ELISA Kit, I-kappaB kinase beta ELISA Kit, inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase beta ELISA Kit, inhibitor of kappaB kinase beta ELISA Kit, ikbkb.L ELISA Kit, IKBKB ELISA Kit, ikbkb ELISA Kit, IKKbeta ELISA Kit, Ikbkb ELISA Kit

Background:

Gene Name: Inhibitor Of Kappa-Light Polypeptide Gene Enhancer In B-Cells Kinase Beta

Gene Aliases: IKK2, NFKBIKB, IKK-beta, IKKB, I-kappa-B kinase 2, Nuclear factor NF-kappa-B inhibitor kinase beta

Gene ID: 3551

UniProt: O14920

Pathways: NF-kappaB Signaling, RTK Signaling, TCR Signaling, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Production of Molecular Mediator of Immune Response, Hepatitis C, Toll-Like Receptors Cascades, BCR Signaling, Ubiquitin Proteasome Pathway, S100 Proteins