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IFNA2 CLIA Kit

IFNA2 Reactivity: Human Chemiluminescent Sandwich ELISA 15.62 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Catalog No. ABIN5655566
  • Target See all IFNA2 CLIA Kits
    IFNA2 (Interferon, alpha 2 (IFNA2))
    Reactivity
    • 3
    • 3
    Human
    Detection Method
    Chemiluminescent
    Method Type
    Sandwich ELISA
    Detection Range
    15.62 pg/mL - 1000 pg/mL
    Minimum Detection Limit
    15.62 pg/mL
    Application
    ELISA
    Sample Type
    Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
    Analytical Method
    Quantitative
    Specificity
    This assay has high sensitivity and excellent specificity for detection of Interferon Alpha 2 (IFNa2). No significant cross-reactivity or interference between Interferon Alpha 2 (IFNa2) and analogues was observed.
    Sensitivity
    0.49 pg/mL
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  • Comment

    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

    Assay Time
    2 - 3 h
    Plate
    Pre-coated
    Protocol
    The microplate provided in this kit has been pre-coated with an antibody specific to Interferon Alpha 2 (IFNa2). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Interferon Alpha 2 (IFNa2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Interferon Alpha 2 (IFNa2) level in the sample or standard.,
    Assay Precision
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Interferon Alpha 2 (IFNa2) were tested 20 times on one plate, respectively
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Interferon Alpha 2 (IFNa2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%
    Restrictions
    For Research Use only
  • Handling Advice
    Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
    Storage
    4 °C,-20 °C
    Storage Comment
    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
    Expiry Date
    4-8 months
  • Target See all IFNA2 CLIA Kits
    IFNA2 (Interferon, alpha 2 (IFNA2))
    Alternative Name
    Interferon Alpha 2 (IFNA2 Products)
    Synonyms
    IFN-alphaA CLIA Kit, IFNA CLIA Kit, IFNA2B CLIA Kit, INFA2 CLIA Kit, Ifa2 CLIA Kit, IFNA2 CLIA Kit, interferon alpha 2 CLIA Kit, interferon, alpha 2 CLIA Kit, interferon-alpha-2 CLIA Kit, interferon alpha-2 CLIA Kit, IFNA2 CLIA Kit, Ifna2 CLIA Kit, ifna2 CLIA Kit, IFN-ALPHA2 CLIA Kit, LOC102142148 CLIA Kit
    Background

    Gene Name: Interferon Alpha 2

    Gene Aliases: IFNA2A, IFNA2B, IFNA2C, LeIF A, Alpha-2a Interferon, Interferon Alpha 2b, Interferon Alpha A

    Gene ID
    3440
    UniProt
    P01563
    Pathways
    JAK-STAT Signaling, Regulation of Leukocyte Mediated Immunity, Production of Molecular Mediator of Immune Response, Hepatitis C
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